Persistent fibrosis, hypertrophy and sarcomere disorganisation after endoscopyguided heart resection in adult Xenopus

Marshall, Lindsey, Vivien, Celine, Girardot, Fabrice, Pericard, Louise, Demeneix, Barbara A., Coen, Laurent and Chai, Norin (2017) Persistent fibrosis, hypertrophy and sarcomere disorganisation after endoscopyguided heart resection in adult Xenopus. PloS One, 12 3: . doi:10.1371/journal.pone.0173418


Author Marshall, Lindsey
Vivien, Celine
Girardot, Fabrice
Pericard, Louise
Demeneix, Barbara A.
Coen, Laurent
Chai, Norin
Title Persistent fibrosis, hypertrophy and sarcomere disorganisation after endoscopyguided heart resection in adult Xenopus
Formatted title
Persistent fibrosis, hypertrophy and sarcomere disorganisation after endoscopyguided heart resection in adult Xenopus
Journal name PloS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2017-03-01
Sub-type Article (original research)
DOI 10.1371/journal.pone.0173418
Open Access Status DOI
Volume 12
Issue 3
Total pages 24
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2018
Language eng
Formatted abstract
Models of cardiac repair are needed to understand mechanisms underlying failure to regenerate in human cardiac tissue. Such studies are currently dominated by the use of zebrafish and mice. Remarkably, it is between these two evolutionary separated species that the adult cardiac regenerative capacity is thought to be lost, but causes of this difference remain largely unknown. Amphibians, evolutionary positioned between these two models, are of particular interest to help fill this lack of knowledge. We thus developed an endoscopy-based resection method to explore the consequences of cardiac injury in adult Xenopus laevis. This method allowed in situ live heart observation, standardised tissue amputation size and reproducibility. During the first week following amputation, gene expression of cell proliferation markers remained unchanged, whereas those relating to sarcomere organisation decreased and markers of inflammation, fibrosis and hypertrophy increased. One-month post-amputation, fibrosis and hypertrophy were evident at the injury site, persisting through 11 months. Moreover, cardiomyocyte sarcomere organisation deteriorated early following amputation, and was not completely recovered as far as 11 months later. We conclude that the adult Xenopus heart is unable to regenerate, displaying cellular and molecular marks of scarring. Our work suggests that, contrary to urodeles and teleosts, with the exception of medaka, adult anurans share a cardiac injury outcome similar to adult mammals. This observation is at odds with current hypotheses that link loss of cardiac regenerative capacity with acquisition of homeothermy.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Article number e0173418

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biomedical Sciences Publications
 
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