Visualization of vascular inflammation in the atherosclerotic mouse by ultrasmall superparamagnetic iron oxide vascular cell adhesion molecule-1-specific nanoparticles

Michalska, Marta, Machtoub, Lina, Manthey, Helga D., Bauer, Elisabeth, Herold, Volker, Krohne, Georg, Lykowsky, Gunthard, Hildenbrand, Markus, Kampf, Thomas, Jakob, Peter, Zernecke, Alma and Bauer, Wolfgang R. (2012) Visualization of vascular inflammation in the atherosclerotic mouse by ultrasmall superparamagnetic iron oxide vascular cell adhesion molecule-1-specific nanoparticles. Arteriosclerosis Thrombosis and Vascular Biology, 32 10: 2350-2357. doi:10.1161/ATVBAHA.112.255224


Author Michalska, Marta
Machtoub, Lina
Manthey, Helga D.
Bauer, Elisabeth
Herold, Volker
Krohne, Georg
Lykowsky, Gunthard
Hildenbrand, Markus
Kampf, Thomas
Jakob, Peter
Zernecke, Alma
Bauer, Wolfgang R.
Title Visualization of vascular inflammation in the atherosclerotic mouse by ultrasmall superparamagnetic iron oxide vascular cell adhesion molecule-1-specific nanoparticles
Journal name Arteriosclerosis Thrombosis and Vascular Biology   Check publisher's open access policy
ISSN 1079-5642
1524-4636
Publication date 2012-10-01
Sub-type Article (original research)
DOI 10.1161/ATVBAHA.112.255224
Open Access Status Not yet assessed
Volume 32
Issue 10
Start page 2350
End page 2357
Total pages 8
Place of publication Philadelphia, PA, United States
Publisher Lippincott Williams & Wilkins
Language eng
Formatted abstract
Objective—Noninvasive imaging of atherosclerosis remains challenging in clinical applications. Here, we applied noninvasive molecular imaging to detect vascular cell adhesion molecule-1 in early and advanced atherosclerotic lesions of apolipoprotein E–deficient mice.

Methods and Results—Ultrasmall superparamagnetic iron oxide particles functionalized with (P03011) or without (P3007) vascular cell adhesion molecule-1−binding peptide were visualized by ultra high-field (17.6 T) magnetic resonance. Injection of P03011 resulted in a marked signal loss in the aortic root of apolipoprotein E–deficient mice fed a Western diet for 8 and 26 weeks in vivo and ex vivo, compared with preinjection measurements, P3007-injected mice, and P03011- or P3007-injected age-matched C57BL/6 controls. Histological analyses revealed iron accumulations in the intima, in colocalization with vascular cell adhesion molecule-1−expressing macrophages and endothelial cells. Coherent anti-Stokes Raman scattering microscopy demonstrated iron signals in the intima and media of the aortic root in the P03011-injected but not untreated apolipoprotein E–deficient mice, localized to macrophages, luminal endothelial-like cells, and medial regions containing smooth muscle cells. Electron microscopy confirmed iron particles enclosed in endothelial cells and in the vicinity of smooth muscle cells.

Conclusion—Using a combination of innovative imaging modalities, in this study, we demonstrate the feasibility of applying P03011 as a contrast agent for imaging of atherosclerosis.
Keyword Atherosclerosis
Iron oxide nanoparticles
Noninvasive imaging
Surface-enhanced coherent anti-Stokes Raman scattering
Vascular cell adhesion molecule-1
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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