Genetically encoded calcium indicators as probes to assess the role of calcium channels in disease and for high-throughput drug discovery

Bassett, J. J. and Monteith, G. R. (2017). Genetically encoded calcium indicators as probes to assess the role of calcium channels in disease and for high-throughput drug discovery. In Ion Channels DownUnder (pp. 141-171) Maryland Heights, MO, United States: Academic Press. doi:10.1016/bs.apha.2017.01.001

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Author Bassett, J. J.
Monteith, G. R.
Title of chapter Genetically encoded calcium indicators as probes to assess the role of calcium channels in disease and for high-throughput drug discovery
Title of book Ion Channels DownUnder
Place of Publication Maryland Heights, MO, United States
Publisher Academic Press
Publication Year 2017
Sub-type Research book chapter (original research)
DOI 10.1016/bs.apha.2017.01.001
Open Access Status Not yet assessed
Year available 2017
Series Advances in Pharmacology
ISBN 9780128104132
ISSN 1557-8925
1054-3589
Volume number 79
Chapter number 5
Start page 141
End page 171
Total pages 31
Total chapters 10
Language eng
Formatted Abstract/Summary
The calcium ion (Ca2 +) is an important signaling molecule implicated in many cellular processes, and the remodeling of Ca2 + homeostasis is a feature of a variety of pathologies. Typical methods to assess Ca2 + signaling in cells often employ small molecule fluorescent dyes, which are sometimes poorly suited to certain applications such as assessment of cellular processes, which occur over long periods (hours or days) or in vivo experiments. Genetically encoded calcium indicators are a set of tools available for the measurement of Ca2 + changes in the cytosol and subcellular compartments, which circumvent some of the inherent limitations of small molecule Ca2 + probes. Recent advances in genetically encoded calcium sensors have greatly increased their ability to provide reliable monitoring of Ca2 + changes in mammalian cells. New genetically encoded calcium indicators have diverse options in terms of targeting, Ca2 + affinity and fluorescence spectra, and this will further enhance their potential use in high-throughput drug discovery and other assays. This review will outline the methods available for Ca2 + measurement in cells, with a focus on genetically encoded calcium sensors. How these sensors will improve our understanding of the deregulation of Ca2 + handling in disease and their application to high-throughput identification of drug leads will also be discussed.
Keyword Genetically encoded calcium indicators
High-throughput screening
Drug discovery
Calcium channels
Calcium signaling
Molecular probes
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status UQ

 
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