A thermosensitive heparin-poloxamer hydrogel bridges aFGF to treat spinal cord injury

Wang, Qingqing, He, Yan, Zhao, Yingzheng, Xie, Huixu, Lin, Qian, He, Zili, Wang, Xiaoyan, Li, Jiawei, Zhang, Hongyu, Wang, Chenggui, Gong, Fanghua, Li, Xiaokun, Xu, Huazi, Ye, Qingsong and Xiao, Jian (2017) A thermosensitive heparin-poloxamer hydrogel bridges aFGF to treat spinal cord injury. ACS Applied Materials and Interfaces, 9 8: 6725-6745. doi:10.1021/acsami.6b13155

Author Wang, Qingqing
He, Yan
Zhao, Yingzheng
Xie, Huixu
Lin, Qian
He, Zili
Wang, Xiaoyan
Li, Jiawei
Zhang, Hongyu
Wang, Chenggui
Gong, Fanghua
Li, Xiaokun
Xu, Huazi
Ye, Qingsong
Xiao, Jian
Title A thermosensitive heparin-poloxamer hydrogel bridges aFGF to treat spinal cord injury
Journal name ACS Applied Materials and Interfaces   Check publisher's open access policy
ISSN 1944-8252
Publication date 2017-03-01
Sub-type Article (original research)
DOI 10.1021/acsami.6b13155
Open Access Status Not yet assessed
Volume 9
Issue 8
Start page 6725
End page 6745
Total pages 21
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Abstract Acidic fibroblast growth factor (aFGF) exerts a protective effect on spinal cord injury (SCI) but is limited by the lack of physicochemical stability and the ability to cross the blood spinal cord barrier (BSCB). As promising biomaterials, hydrogels contain substantial amounts of water and a three-dimensional porous structure and are commonly used to load and deliver growth factors. Heparin can not only enhance growth factor loading onto hydrogels but also can stabilize the structure and control the release behavior. Herein, a novel aFGF-loaded thermosensitive heparin-poloxamer (aFGF-HP) hydrogel was developed and applied to provide protection and regeneration after SCI. To assess the effects of the aFGF-HP hydrogel, BSCB restoration, neuron and axonal rehabilitation, glial scar inhibition, inflammatory response suppression, and motor recovery were studied both in vivo and in vitro. The aFGF-HP hydrogels exhibited sustained release of aFGF and protected the bioactivity of aFGF in vitro. Compared to groups intravenously administered either drug-free HP hydrogel or aFGF alone, the aFGF-HP hydrogel group revealed prominent and attenuated disruption of the BSCB, reduced neuronal apoptosis, reactive astrogliosis, and increased neuron and axonal rehabilitation both in vivo and in vitro. This work provides an effective approach to enhance recovery after SCI and provide a successful strategy for SCI protection.
Keyword Acidic fibroblast growth factor
Control release
Spinal cord injury
Thermosensitive hydrogel
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
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