High levels of human antigen-specific CD4+ T cells in peripheral blood revealed by stimulated coexpression of CD25 and CD134 (OX40)

Zaunders, John J., Munier, Mee Ling, Seddiki, Nabila, Pett, Sarah, Ip, Susanna, Bailey, Michelle, Xu, Yin, Brown, Kai, Dyer, Wayne B., Kim, Min, de Rose, Robert, Kent, Stephen J., Jiang, Lele, Breit, Samuel N., Emery, Sean, Cunningham, Anthony L., Cooper, David A. and Kelleher, Anthony D. (2009) High levels of human antigen-specific CD4+ T cells in peripheral blood revealed by stimulated coexpression of CD25 and CD134 (OX40). Journal of Immunology, 183 4: 2827-2836. doi:10.4049/jimmunol.0803548


Author Zaunders, John J.
Munier, Mee Ling
Seddiki, Nabila
Pett, Sarah
Ip, Susanna
Bailey, Michelle
Xu, Yin
Brown, Kai
Dyer, Wayne B.
Kim, Min
de Rose, Robert
Kent, Stephen J.
Jiang, Lele
Breit, Samuel N.
Emery, Sean
Cunningham, Anthony L.
Cooper, David A.
Kelleher, Anthony D.
Title High levels of human antigen-specific CD4+ T cells in peripheral blood revealed by stimulated coexpression of CD25 and CD134 (OX40)
Formatted title
High levels of human antigen-specific CD4+ T cells in peripheral blood revealed by stimulated coexpression of CD25 and CD134 (OX40)
Journal name Journal of Immunology   Check publisher's open access policy
ISSN 0022-1767
1550-6606
Publication date 2009-08-15
Sub-type Article (original research)
DOI 10.4049/jimmunol.0803548
Open Access Status Not yet assessed
Volume 183
Issue 4
Start page 2827
End page 2836
Total pages 10
Place of publication Bethesda, MD, United States
Publisher American Association of Immunologists
Language eng
Formatted abstract
Ag-specific human CD4+ memory T lymphocytes have mostly been studied using assays of proliferation in vitro. Intracellular cytokine and ELISPOT assays quantify effector cell populations but barely detect responses to certain recall Ags that elicit strong proliferative responses, e.g., tetanus toxoid, that comprise non-Th1 CD4+ cells. We have found that culturing whole blood with Ag for 40-48 h induces specific CD4+ T cells to simultaneously express CD25 and CD134. This new technique readily detects responses to well-described CD4+ T cell recall Ags, including preparations of mycobacteria, CMV, HSV-1, influenza, tetanus toxoid, Candida albicans, and streptokinase, as well as HIV-1 peptides, with high specificity. The assay detects much higher levels of Ag-specific cells than intracellular cytokine assays, plus the cells retain viability and can be sorted for in vitro expansion. Furthermore, current in vitro assays for human CD4+ memory T lymphocytes are too labor-intensive and difficult to standardize for routine diagnostic laboratories, whereas the whole-blood CD25+CD134+ assay combines simplicity of setup with a straight-forward cell surface flow cytometry readout. In addition to revealing the true extent of Ag-specific human CD4+ memory T lymphocytes, its greatest use will be as a simple in vitro monitor of CD4+ T cell responses to Ags such as tuberculosis infection or vaccines.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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