Visualization of the reaction trajectory and transition state in a hydrolytic reaction catalyzed by a metalloenzyme

Selleck, Christopher, Clayton, Daniel, Gahan, Lawrence R., Mitic, Nataša, McGeary, Ross P., Pedroso, Marcelo Monteiro, Guddat, Luke W. and Schenk, Gerhard (2017) Visualization of the reaction trajectory and transition state in a hydrolytic reaction catalyzed by a metalloenzyme. Chemistry: A European Journal, 23 20: 4778-4781. doi:10.1002/chem.201700866


Author Selleck, Christopher
Clayton, Daniel
Gahan, Lawrence R.
Mitic, Nataša
McGeary, Ross P.
Pedroso, Marcelo Monteiro
Guddat, Luke W.
Schenk, Gerhard
Title Visualization of the reaction trajectory and transition state in a hydrolytic reaction catalyzed by a metalloenzyme
Journal name Chemistry: A European Journal   Check publisher's open access policy
ISSN 1521-3765
0947-6539
Publication date 2017-03-05
Year available 2017
Sub-type Article (original research)
DOI 10.1002/chem.201700866
Open Access Status Not yet assessed
Volume 23
Issue 20
Start page 4778
End page 4781
Total pages 5
Place of publication Weinheim, Germany
Publisher Wiley
Collection year 2018
Language eng
Formatted abstract
Metallohydrolases are a vast family of enzymes that play crucial roles in numerous metabolic pathways. Several members have emerged as targets for chemotherapeutics. Knowledge about their reaction mechanisms and associated transition states greatly aids the design of potent and highly specific drug leads. Using a high-resolution crystal structure we have probed the trajectory of the reaction catalyzed by purple acid phosphatase, an enzyme essential for the integrity of bone structure. In particular the transition state is visualized, thus providing detailed structural information that may be exploited in the design of specific inhibitors for the development of novel anti-osteoporotic chemotherapeutics.
Keyword Metallohydrolases
Phosphate coordination
transition state
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Fri, 17 Mar 2017, 13:44:04 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences