Selective anti-proliferative activities of Carica papaya leaf juice extracts against prostate cancer

Pandey, Saurabh, Walpole, Carina, Cabot, Peter J., Shaw, Paul N., Batra, Jyotsna and Hewavitharana, Amitha K. (2017) Selective anti-proliferative activities of Carica papaya leaf juice extracts against prostate cancer. Biomedicine & Pharmacotherapy, 89 515-523. doi:10.1016/j.biopha.2017.02.050


Author Pandey, Saurabh
Walpole, Carina
Cabot, Peter J.
Shaw, Paul N.
Batra, Jyotsna
Hewavitharana, Amitha K.
Title Selective anti-proliferative activities of Carica papaya leaf juice extracts against prostate cancer
Formatted title
Selective anti-proliferative activities of Carica papaya leaf juice extracts against prostate cancer
Journal name Biomedicine & Pharmacotherapy   Check publisher's open access policy
ISSN 1950-6007
0753-3322
Publication date 2017-05-01
Sub-type Article (original research)
DOI 10.1016/j.biopha.2017.02.050
Open Access Status Not yet assessed
Volume 89
Start page 515
End page 523
Total pages 9
Place of publication Cedex, France
Publisher Elsevier Masson
Collection year 2018
Language eng
Formatted abstract
Background: Prostate cancer (PCa) is the leading cause of cancer related deaths in men. Carica papaya is a popular tropical plant that has been traditionally used for its nutritional and medicinal properties.

Methods: We investigated the anti-proliferative responses of papaya leaf juice (LJP) and its various extracts (“biological”- in vitro digested, “physical”- size exclusion, and “chemical”-solvent extraction) on a range of cell lines representing benign hyperplasia, tumorigenic and normal cells of prostate origin.

Results: Time course analysis (by 24 h, 48 h and 72 h) of LJP (1–0.1 mg/mL) before and after in vitro digestion, and of molecular weight based fractions of LJP showed anti-proliferative responses. The medium polarity fraction of LJP (0.03–0.003 mg/mL) after 72 h exposure showed potent growth inhibitory (IC50 = 0.02–0.07 mg/mL) and cytotoxic activities on all prostate cells, with the exception of the normal (RWPE-1 and WPMY-1) cells. Flow cytometry analysis showed S phase cell cycle arrest and apoptosis as a possible mechanism for these activities. Medium polar fraction of LJP also inhibited migration and adhesion of metastatic PC-3 cells.

Conclusion: This is the first report suggesting selective anti-proliferative and anti-metastatic attributes of LJP extract against prostatic diseases, including PCa.
Keyword Anti-proliferative activity
Extraction
Papaya
Prostate cancer
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Pharmacy Publications
 
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