Selective anti-proliferative activities of Carica papaya leaf juice extracts against prostate cancer

Pandey, Saurabh, Walpole, Carina, Cabot, Peter J., Shaw, Paul N., Batra, Jyotsna and Hewavitharana, Amitha K. (2017) Selective anti-proliferative activities of Carica papaya leaf juice extracts against prostate cancer. Biomedicine & Pharmacotherapy, 89 515-523. doi:10.1016/j.biopha.2017.02.050


Author Pandey, Saurabh
Walpole, Carina
Cabot, Peter J.
Shaw, Paul N.
Batra, Jyotsna
Hewavitharana, Amitha K.
Title Selective anti-proliferative activities of Carica papaya leaf juice extracts against prostate cancer
Formatted title
Selective anti-proliferative activities of Carica papaya leaf juice extracts against prostate cancer
Journal name Biomedicine & Pharmacotherapy   Check publisher's open access policy
ISSN 1950-6007
0753-3322
Publication date 2017-05-01
Year available 2017
Sub-type Article (original research)
DOI 10.1016/j.biopha.2017.02.050
Open Access Status Not yet assessed
Volume 89
Start page 515
End page 523
Total pages 9
Place of publication Cedex, France
Publisher Elsevier Masson
Language eng
Subject 3004 Pharmacology
Abstract Background Prostate cancer (PCa) is the leading cause of cancer related deaths in men. Carica papaya is a popular tropical plant that has been traditionally used for its nutritional and medicinal properties. Methods We investigated the anti-proliferative responses of papaya leaf juice (LJP) and its various extracts (“biological”- in vitro digested, “physical”- size exclusion, and “chemical”-solvent extraction) on a range of cell lines representing benign hyperplasia, tumorigenic and normal cells of prostate origin. Results Time course analysis (by 24 h, 48 h and 72 h) of LJP (1–0.1 mg/mL) before and after in vitro digestion, and of molecular weight based fractions of LJP showed anti-proliferative responses. The medium polarity fraction of LJP (0.03–0.003 mg/mL) after 72 h exposure showed potent growth inhibitory (IC = 0.02–0.07 mg/mL) and cytotoxic activities on all prostate cells, with the exception of the normal (RWPE-1 and WPMY-1) cells. Flow cytometry analysis showed S phase cell cycle arrest and apoptosis as a possible mechanism for these activities. Medium polar fraction of LJP also inhibited migration and adhesion of metastatic PC-3 cells. Conclusion This is the first report suggesting selective anti-proliferative and anti-metastatic attributes of LJP extract against prostatic diseases, including PCa.
Formatted abstract
Background: Prostate cancer (PCa) is the leading cause of cancer related deaths in men. Carica papaya is a popular tropical plant that has been traditionally used for its nutritional and medicinal properties.

Methods: We investigated the anti-proliferative responses of papaya leaf juice (LJP) and its various extracts (“biological”- in vitro digested, “physical”- size exclusion, and “chemical”-solvent extraction) on a range of cell lines representing benign hyperplasia, tumorigenic and normal cells of prostate origin.

Results: Time course analysis (by 24 h, 48 h and 72 h) of LJP (1–0.1 mg/mL) before and after in vitro digestion, and of molecular weight based fractions of LJP showed anti-proliferative responses. The medium polarity fraction of LJP (0.03–0.003 mg/mL) after 72 h exposure showed potent growth inhibitory (IC50 = 0.02–0.07 mg/mL) and cytotoxic activities on all prostate cells, with the exception of the normal (RWPE-1 and WPMY-1) cells. Flow cytometry analysis showed S phase cell cycle arrest and apoptosis as a possible mechanism for these activities. Medium polar fraction of LJP also inhibited migration and adhesion of metastatic PC-3 cells.

Conclusion: This is the first report suggesting selective anti-proliferative and anti-metastatic attributes of LJP extract against prostatic diseases, including PCa.
Keyword Anti-proliferative activity
Extraction
Papaya
Prostate cancer
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Pharmacy Publications
 
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