Non-invasive metabolic imaging of melanoma progression

Pastore, Michael N., Studier, Hauke, Bonder, Claudine S. and Roberts, Michael S. (2017) Non-invasive metabolic imaging of melanoma progression. Experimental Dermatology, 26 7: 607-614. doi:10.1111/exd.13274

Author Pastore, Michael N.
Studier, Hauke
Bonder, Claudine S.
Roberts, Michael S.
Title Non-invasive metabolic imaging of melanoma progression
Journal name Experimental Dermatology   Check publisher's open access policy
ISSN 1600-0625
Publication date 2017-01-01
Sub-type Article (original research)
DOI 10.1111/exd.13274
Open Access Status Not yet assessed
Volume 26
Issue 7
Start page 607
End page 614
Total pages 8
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Abstract Skin cancer is associated with abnormal cellular metabolism which if identified early introduces the possibility of intervention to prevent its progress to a deadly metastatic stage. This study combines multiphoton microscopy with fluorescence lifetime imaging (FLIM) using a syngeneic melanoma mouse model, to detect changes in metabolic state of single epidermal cells as a metabolic marker to monitor the progress of tumor growth. This method utilizes imaging of the ratio of the amounts of the free and protein-bound forms of the intracellular autofluorescent metabolic co-enzyme nicotinamide adenine dinucleotide (NADH). Here, we investigate the impact of the primary tumor lesion on the epidermal layers at three different growth stages of melanoma lesion compared to normal skin as a control. We showed a significant increase in the free-to-bound NADH ratio with the growth of the solid melanoma tumor, while concurrently the short and the long lifetime components of NADH remained constant. These results demonstrate the ability of FLIM for rapid, non-invasive and sensitive assessment of melanoma progression revealing its potential as a diagnostic tool for melanoma detection and as an aid for melanoma staging.
Keyword NADH
Fluorescence lifetime imaging microscopy
Metabolic imaging
Mouse model
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
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