Amino acid transporter mutants of Arabidopsis provides evidence that a non-mycorrhizal plant acquires organic nitrogen from agricultural soil

Ganeteg, Ulrika, Ahmad, Iftikhar, Jamtgard, Sandra, Aguetoni-Cambui, Camila, Inselsbacher, Erich, Svennerstam, Henrik, Schmidt, Susanne and Nasholm, Torgny (2017) Amino acid transporter mutants of Arabidopsis provides evidence that a non-mycorrhizal plant acquires organic nitrogen from agricultural soil. Plant, Cell and Environment, 40 3: 413-423. doi:10.1111/pce.12881


Author Ganeteg, Ulrika
Ahmad, Iftikhar
Jamtgard, Sandra
Aguetoni-Cambui, Camila
Inselsbacher, Erich
Svennerstam, Henrik
Schmidt, Susanne
Nasholm, Torgny
Title Amino acid transporter mutants of Arabidopsis provides evidence that a non-mycorrhizal plant acquires organic nitrogen from agricultural soil
Formatted title
Amino acid transporter mutants of Arabidopsis provides evidence that a non-mycorrhizal plant acquires organic nitrogen from agricultural soil
Journal name Plant, Cell and Environment   Check publisher's open access policy
ISSN 1365-3040
0140-7791
Publication date 2017-03-01
Sub-type Article (original research)
DOI 10.1111/pce.12881
Open Access Status Not yet assessed
Volume 40
Issue 3
Start page 413
End page 423
Total pages 11
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Although organic nitrogen (N) compounds are ubiquitous in soil solutions, their potential role in plant N nutrition has been questioned. We performed a range of experiments on Arabidopsis thaliana genetically modified to enhance or reduce root uptake of amino acids. Plants lacking expression of the Lysine Histidine Transporter 1 (LHT1) displayed significantly lower contents of 13C and 15N label and of U-13C5,15N2 L-glutamine, as determined by liquid chromatography–mass spectrometry when growing in pots and supplied with dually labelled L-glutamine compared to wild type plants and LHT1-overexpressing plants. Slopes of regressions between accumulation of 13C-labelled carbon and 15N-labelled N were higher for LHT1-overexpressing plants than wild type plants, while plants lacking expression of LHT1 did not display a significant regression between the two isotopes. Uptake of labelled organic N from soil tallied with that of labelled ammonium for wild type plants and LHT1-overexpressing plants but was significantly lower for plants lacking expression of LHT1. When grown on agricultural soil plants lacking expression of LHT1 had the lowest, and plants overexpressing LHT1 the highest C/N ratios and natural δ15N abundance suggesting their dependence on different N pools. Our data show that LHT1 expression is crucial for plant uptake of organic N from soil.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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