Development of a human vasopressin V1a-receptor antagonist from an evolutionary-related insect neuropeptide

Di Giglio, Maria Giulia , Muttenthaler, Markus, Harpsoe, Kasper, Liutkeviciute, Zita, Keov, Peter, Eder, Thomas, Rattei, Thomas, Arrowsmith, Sarah, Wray, Susan, Marek, Ales, Elbert, Tomas, Alewood, Paul F. , Gloriam, David E. and Gruber, Christian W. (2017) Development of a human vasopressin V1a-receptor antagonist from an evolutionary-related insect neuropeptide. Scientific Reports, 7 . doi:10.1038/srep41002


Author Di Giglio, Maria Giulia
Muttenthaler, Markus
Harpsoe, Kasper
Liutkeviciute, Zita
Keov, Peter
Eder, Thomas
Rattei, Thomas
Arrowsmith, Sarah
Wray, Susan
Marek, Ales
Elbert, Tomas
Alewood, Paul F.
Gloriam, David E.
Gruber, Christian W.
Title Development of a human vasopressin V1a-receptor antagonist from an evolutionary-related insect neuropeptide
Formatted title
Development of a human vasopressin V1a-receptor antagonist from an evolutionary-related insect neuropeptide
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2017-02-01
Sub-type Article (original research)
DOI 10.1038/srep41002
Open Access Status DOI
Volume 7
Total pages 15
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Formatted abstract
Characterisation of G protein-coupled receptors (GPCR) relies on the availability of a toolbox of ligands that selectively modulate different functional states of the receptors. To uncover such molecules, we explored a unique strategy for ligand discovery that takes advantage of the evolutionary conservation of the 600-million-year-old oxytocin/vasopressin signalling system. We isolated the insect oxytocin/vasopressin orthologue inotocin from the black garden ant (Lasius Niger), identified and cloned its cognate receptor and determined its pharmacological properties on the insect and human oxytocin/vasopressin receptors. Subsequently, we identified a functional dichotomy: inotocin activated the insect inotocin and the human vasopressin V1b receptors, but inhibited the human V1aR. Replacement of Arg8 of inotocin by D-Arg8 led to a potent, stable and competitive V1aR-Antagonist ([D-Arg8]-inotocin) with a 3,000-fold binding selectivity for the human V1aR over the other three subtypes, OTR, V1bR and V2R. The Arg8/D-Arg8 ligand-pair was further investigated to gain novel insights into the oxytocin/vasopressin peptide-receptor interaction, which led to the identification of key residues of the receptors that are important for ligand functionality and selectivity. These observations could play an important role for development of oxytocin/vasopressin receptor modulators that would enable clear distinction of the physiological and pathological responses of the individual receptor subtypes.
Keyword Entomology
Peptides
Receptor pharmacology
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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