Effect of standard tuberculosis treatment on plasma cytokine levels in patients with active pulmonary tuberculosis

Riou, Catherine, Perez Peixoto, Blas, Roberts, Lindi, Ronacher, Katharina, Walzl, Gerhard, Manca, Claudia, Rustomjee, Roxana, Mthiyane, Thuli, Fallows, Dorothy, Gray, Clive M. and Kaplan, Gilla (2012) Effect of standard tuberculosis treatment on plasma cytokine levels in patients with active pulmonary tuberculosis. PLoS One, 7 5: 123-137. doi:10.1371/journal.pone.0036886

Author Riou, Catherine
Perez Peixoto, Blas
Roberts, Lindi
Ronacher, Katharina
Walzl, Gerhard
Manca, Claudia
Rustomjee, Roxana
Mthiyane, Thuli
Fallows, Dorothy
Gray, Clive M.
Kaplan, Gilla
Title Effect of standard tuberculosis treatment on plasma cytokine levels in patients with active pulmonary tuberculosis
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
ISBN 978-1-57808-690-0
Publication date 2012-05-14
Year available 2012
Sub-type Article (original research)
DOI 10.1371/journal.pone.0036886
Open Access Status DOI
Volume 7
Issue 5
Start page 123
End page 137
Total pages 12
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Language eng
Formatted abstract

Sputum Mycobacterium tuberculosis (Mtb) culture is commonly used to assess response to antibiotic treatment in individuals with pulmonary tuberculosis (TB). Such techniques are constrained by the slow growth rate of Mtb, and more sensitive methods to monitor Mtb clearance are needed. The goal of this study was to evaluate changes in plasma cytokines in patients undergoing treatment for TB as a means of identifying candidate host markers associated with microbiologic response to therapy.


Twenty-four plasma cytokines/chemokines were measured in 42 individuals diagnosed with active pulmonary TB, 52% were HIV co-infected. Individuals, undergoing a 26-week standard TB treatment, were followed longitudinally over 18 months and measurements were associated with HIV status and rates of sputum culture conversion.


Plasma concentrations of interferon-inducible protein-10 (IP-10) and vascular endothelial growth factor (VEGF) were significantly reduced upon TB treatment, regardless of HIV status. By the end of treatment, IP-10 concentrations were significantly lower in HIV negative individuals when compared to HIV-positive individuals (pā€Š=ā€Š0.02). Moreover, in HIV negative patients, plasma VEGF concentrations, measured as early as 2-weeks post TB treatment initiation, positively correlated with the time of sputum conversion (pā€Š=ā€Š0.0017). No significant changes were observed in other studied immune mediators.


These data suggest that VEGF plasma concentration, measured during early TB treatment, could represent a surrogate marker to monitor sputum culture conversion in HIV uninfected individuals.
Keyword Multidisciplinary Sciences
Science & Technology - Other Topics
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID RO1-AI080737
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Mater Research Institute-UQ (MRI-UQ)
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Citation counts: TR Web of Science Citation Count  Cited 38 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 16 Feb 2017, 11:34:13 EST by Johanna Barclay on behalf of Mater Research Institute-UQ