Autophagic vesicles on mature human reticulocytes explain phosphatidylserine-positive red cells in sickle cell disease

Mankelow, Tosti J., Griffiths, Rebecca E., Trompeter, Sara, Flatt, Joanna F., Cogan, Nicola M., Massey, Edwin J. and Anstee, David J. (2015) Autophagic vesicles on mature human reticulocytes explain phosphatidylserine-positive red cells in sickle cell disease. Blood, 126 15: 1831-1834. doi:10.1182/blood-2015-04-637702


Author Mankelow, Tosti J.
Griffiths, Rebecca E.
Trompeter, Sara
Flatt, Joanna F.
Cogan, Nicola M.
Massey, Edwin J.
Anstee, David J.
Title Autophagic vesicles on mature human reticulocytes explain phosphatidylserine-positive red cells in sickle cell disease
Journal name Blood   Check publisher's open access policy
ISSN 1528-0020
0006-4971
Publication date 2015-10-01
Sub-type Article (original research)
DOI 10.1182/blood-2015-04-637702
Open Access Status Not Open Access
Volume 126
Issue 15
Start page 1831
End page 1834
Total pages 4
Place of publication Washington, DC, United States
Publisher American Society of Hematology
Abstract During maturation to an erythrocyte, a reticulocyte must eliminate any residual organelles andreduce itssurface area andvolume. Hereweshowthis involves anovel processwhereby large, intact, inside-out phosphatidylserine (PS)-exposed autophagic vesicles are extruded. Cell surface PS is awell-characterized apoptotic signal initiating phagocytosis. In peripheral blood from patients after splenectomy or in patients with sickle cell disease (SCD), the number of circulating red cells exposing PS on their surface is elevated. We show that in these patientsPS is presentonthe cell surfaceof red cells inlarge (∼1.4 mm) discrete areas corresponding to autophagic vesicles. The autophagic vesicles found on reticulocytes are identical to those observed on red cells from splenectomized individuals and patients with SCD. Our data suggest the increased thrombotic risk associated with splenectomy, and patients with hemoglobinopathies is a possible consequence of increased levels of circulating mature reticulocytes expressing inside-out PS-exposed autophagic vesicles because of asplenia.
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID RP-PG-0310-1004
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Tue, 14 Feb 2017, 14:47:00 EST by Rebecca Griffiths on behalf of Learning and Research Services (UQ Library)