si-RNA-mediated silencing of ADRBK1 gene attenuates breast cancer cell proliferation

Zhang, Chen, Chen, Xianzhen, Li, Yongxin, S. W. A., Himaya, Wu, Jie, Shi, Xiujuan, Liu, Xiaoqing and Kim, Sekwon (2014) si-RNA-mediated silencing of ADRBK1 gene attenuates breast cancer cell proliferation. Cancer Biotherapy and Radiopharmaceuticals, 29 8: 303-309. doi:10.1089/cbr.2014.1653

Author Zhang, Chen
Chen, Xianzhen
Li, Yongxin
S. W. A., Himaya
Wu, Jie
Shi, Xiujuan
Liu, Xiaoqing
Kim, Sekwon
Title si-RNA-mediated silencing of ADRBK1 gene attenuates breast cancer cell proliferation
Journal name Cancer Biotherapy and Radiopharmaceuticals   Check publisher's open access policy
ISSN 1557-8852
Publication date 2014-10-03
Year available 2014
Sub-type Article (original research)
DOI 10.1089/cbr.2014.1653
Open Access Status Not yet assessed
Volume 29
Issue 8
Start page 303
End page 309
Total pages 7
Place of publication New Rochelle, NY, United States
Publisher Mary Ann Liebert
Language eng
Formatted abstract
Breast cancer is the most prominent cause of cancer-related deaths among women worldwide. It has been found that genetic mutations play distinct roles in the onset and progression of breast cancer. Androgenic, beta, receptor kinase 1 (ADRBK1) has been reported to possess oncogenic characteristics vital for cancer cell viability. This study was designed to investigate the effects of small interference RNA (si-RNA)-mediated ADRBK1 knockdown on breast cancer cell growth in vitro. High-expression levels of ADRBK1 were observed in all tested breast cancer cell lines (MDA-MB-231, MCF-7, T-47D, and BT-474). ADRBK1 si-RNA was delivered to breast cancer cells using lentivirus delivery system. Depletion of ADRBK1 significantly attenuated the cell viability and colony-formation ability. Flow cytometry analysis further demonstrated that ADRBK1 silencing led to MDA-MB-231 cell arrest in the G0/G1 phase. Collectively, these results indicate that knockdown of ADRBK1 gene has detrimental effects on breast cancer cell growth, which may be a potential therapeutic approach for the treatment of breast cancer.
Keyword ADRBK1
Breast cancer
Cell growth
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 81100673
2013 M541548
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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