Increased synaptic excitation and abnormal dendritic structure of prefrontal cortex layer V pyramidal neurons following prolonged binge-like consumption of ethanol

Klenowski, Paul M., Fogarty, Matthew J., Shariff, Masroor, Belmer, Arnauld, Bellingham, Mark C. and Bartlett, Selena E. (2016) Increased synaptic excitation and abnormal dendritic structure of prefrontal cortex layer V pyramidal neurons following prolonged binge-like consumption of ethanol. eNeuro, 3 6: . doi:10.1523/eneuro.0248-16.2016


Author Klenowski, Paul M.
Fogarty, Matthew J.
Shariff, Masroor
Belmer, Arnauld
Bellingham, Mark C.
Bartlett, Selena E.
Title Increased synaptic excitation and abnormal dendritic structure of prefrontal cortex layer V pyramidal neurons following prolonged binge-like consumption of ethanol
Journal name eNeuro   Check publisher's open access policy
ISSN 2373-2822
Publication date 2016-11-30
Year available 2016
Sub-type Article (original research)
DOI 10.1523/eneuro.0248-16.2016
Open Access Status DOI
Volume 3
Issue 6
Total pages 15
Place of publication Washington, DC, United States
Publisher Society for Neuroscience
Language eng
Abstract Long-term alcohol use causes a multitude of neurochemical changes in cortical regions that facilitate the transition to dependence. Therefore, we used a model of long-term, binge-like ethanol consumption in rats to determine the effects on morphology and synaptic physiology of medial prefrontal cortex (mPFC) layer V pyramidal neurons. Following 10 weeks of ethanol consumption, we recorded synaptic currents from mPFC neurons and used neurobiotin filling to analyze their morphology. We then compared these data to measurements obtained from age-matched, water-drinking control rats. We found that long-term ethanol consumption caused a significant increase in total dendrite arbor length of mPFC layer V pyramidal neurons. Dendritic restructuring was primarily observed in basal dendrite arbors, with mPFC neurons from animals engaged in long-term ethanol drinking having significantly larger and more complex basal arbors compared with controls. These changes were accompanied by significantly increased total spine densities and spontaneous postsynaptic excitatory current frequency, suggesting that long-term binge-like ethanol consumption enhances basal excitatory synaptic transmission in mPFC layer V pyramidal neurons. Our results provide insights into the morphological and functional changes in mPFC layer V pyramidal neuronal physiology following prolonged exposure to ethanol and support changes in mPFC activity during the development of alcohol dependence.
Keyword Dendrite
Ethanol
MPFC
Pyramidal
Spines
Synaptic currents
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 1061979
FT1110884
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biomedical Sciences Publications
 
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Created: Mon, 06 Feb 2017, 13:22:24 EST by Dr Mark Bellingham on behalf of School of Biomedical Sciences