Identification of coagulase-negative staphylococci from bovine intramammary infection by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Tomazi, Tiago, Goncalves, Juliano Leonel, Barreiro, Juliana Regina, De Campos Braga, Patrícia Aparecida, Prada E Silva, Luis Felipe, Eberlin, Marcos Nogueira and Dos Santos, Marcos Veiga (2014) Identification of coagulase-negative staphylococci from bovine intramammary infection by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Journal of Clinical Microbiology, 52 5: 1658-1663. doi:10.1128/JCM.03032-13

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Author Tomazi, Tiago
Goncalves, Juliano Leonel
Barreiro, Juliana Regina
De Campos Braga, Patrícia Aparecida
Prada E Silva, Luis Felipe
Eberlin, Marcos Nogueira
Dos Santos, Marcos Veiga
Title Identification of coagulase-negative staphylococci from bovine intramammary infection by matrix-assisted laser desorption ionization-time of flight mass spectrometry
Journal name Journal of Clinical Microbiology   Check publisher's open access policy
ISSN 1098-660X
Publication date 2014-05-01
Year available 2014
Sub-type Article (original research)
DOI 10.1128/JCM.03032-13
Open Access Status DOI
Volume 52
Issue 5
Start page 1658
End page 1663
Total pages 6
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract Coagulase-negative staphylococci (CoNS) are among the main pathogens causing bovine intramammary infection (IMI) in many countries. However, one of the limitations related to the specific diagnosis of CoNS is the lack of an accurate, rapid, and convenient method that can differentiate the bacterial species comprising this group. The aim of this study was to evaluate the ability of matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) to accurately identify CoNS species in dairy cow IMI. In addition, the study aimed to determine the frequency of CoNS species causing bovine IMI. A total of 108 bacterial isolates were diagnosed as CoNS by microbiological cultures from two milk samples collected from 21 dairy herds; the first sample was collected at the cow level (i.e., 1,242 composite samples from all quarters), while the second sample was collected at the mammary quarter level (i.e., 1,140 mammary samples collected from 285 cows). After CoNS isolation was confirmed by microbiological culture for both samples, all CoNS isolates (n = 108) were genotypically differentiated by PCR restriction fragment length polymorphism (RFLP) analysis of a partial groEL gene sequence and subjected to the MALDI-TOF MS identification procedure. MALDI-TOF MS correctly identified 103 (95.4%) of the CoNS isolates identified by PCR-RFLP at the species level. Eleven CoNS species isolated from bovine IMI were identified by PCR-RFLP, and the most prevalent species was Staphylococcus chromogenes (n = 80; 74.1%). In conclusion, MALDI-TOF MS may be a reliable alternative method for differentiating CoNS species causing bovine IMI.
Keyword High resolution
MALDI
Mass spectrometry imaging
Matrix
Spatial metabolomics
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 2011/04063-1
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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