Investigating the effect of substrate materials on wearable immunoassay performance

Lee, Khai T., Coffey, Jacob W., Robinson, Kye J., Muller, David A., Grondahl, Lisbeth, Kendall, Mark A. F., Young, Paul R. and Corrie, Simon R. (2017) Investigating the effect of substrate materials on wearable immunoassay performance. Langmuir, 33 3: 773-782. doi:10.1021/acs.langmuir.6b03933

Author Lee, Khai T.
Coffey, Jacob W.
Robinson, Kye J.
Muller, David A.
Grondahl, Lisbeth
Kendall, Mark A. F.
Young, Paul R.
Corrie, Simon R.
Title Investigating the effect of substrate materials on wearable immunoassay performance
Journal name Langmuir   Check publisher's open access policy
ISSN 1520-5827
Publication date 2017-01-24
Year available 2017
Sub-type Article (original research)
DOI 10.1021/acs.langmuir.6b03933
Open Access Status Not yet assessed
Volume 33
Issue 3
Start page 773
End page 782
Total pages 10
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Subject 2500 Materials Science
3104 Condensed Matter Physics
3110 Surfaces and Interfaces
1607 Spectroscopy
1603 Electrochemistry
Abstract Immunoassays are ubiquitous across research and clinical laboratories, yet little attention is paid to the effect of the substrate material on the assay performance characteristics. Given the emerging interest in wearable immunoassay formats, investigations into substrate materials that provide an optimal mix of mechanical and bioanalytical properties are paramount. In the course of our research in developing wearable immunoassays which can penetrate skin to selectively capture disease antigens from the underlying blood vessels, we recently identified significant differences in immunoassay performance between gold and polycarbonate surfaces, even with a consistent surface modification procedure. We observed significant differences in PEG density, antibody immobilization, and nonspecific adsorption between the two substrates. Despite a higher PEG density formed on gold-coated surfaces than on aminefunctionalized polycarbonate, the latter revealed a higher immobilized capture antibody density and lower nonspecific adsorption, leading to improved signal-to-noise ratios and assay sensitivities. The major conclusion from this study is that in designing wearable bioassays or biosensors, the design and its effect on the antifouling polymer layer can significantly affect the assay performance in terms of analytical specificity and sensitivity.
Keyword Self-Assembled Monolayers
Chain Density
Microprojection Arrays
Poly(Ethylene Glycol)
Protein Resistance
Nonspecific Adsorption
Neutron Reflectometry
Biomarker Capture
Grafting Density
Ns1 Protein
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID APP1075739
Institutional Status UQ

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