Chicken muscle mitochondrial content appears co-ordinately regulated and is associated with performance phenotypes

Reverter, Antonio, Okimoto, Ron, Sapp, Robyn, Bottje, Walter G., Hawken, Rachel and Hudson, Nicholas J. (2017) Chicken muscle mitochondrial content appears co-ordinately regulated and is associated with performance phenotypes. Biology Open, 6 1: 50-58. doi:10.1242/bio.022772


Author Reverter, Antonio
Okimoto, Ron
Sapp, Robyn
Bottje, Walter G.
Hawken, Rachel
Hudson, Nicholas J.
Title Chicken muscle mitochondrial content appears co-ordinately regulated and is associated with performance phenotypes
Journal name Biology Open   Check publisher's open access policy
ISSN 2046-6390
Publication date 2017-01-15
Year available 2016
Sub-type Article (original research)
DOI 10.1242/bio.022772
Open Access Status DOI
Volume 6
Issue 1
Start page 50
End page 58
Total pages 9
Place of publication Cambridge, United Kingdom
Publisher Company of Biologists
Language eng
Abstract Mitochondrial content is a fundamental cellular bioenergetic phenotype. Previous work has hypothesised possible links between variation in muscle mitochondrial content and animal performance. However, no population screens have been performed in any production species. Here, we have designed a high throughput molecular approach to estimate mitochondrial content in commercial broilers. Technical validity was established using several approaches, including its performance in monoclonal DF-1 cells, cross-tissue comparisons in tissues with differing metabolic demands (white fat
Formatted abstract
Mitochondrial content is a fundamental cellular bioenergetic phenotype. Previous work has hypothesised possible links between variation in muscle mitochondrial content and animal performance. However, no population screens have been performed in any production species. Here, we have designed a high throughput molecular approach to estimate mitochondrial content in commercial broilers. Technical validity was established using several approaches, including its performance in monoclonal DF-1 cells, cross-tissue comparisons in tissues with differing metabolic demands (white fat<breast muscle<drumstick muscle<heart muscle) and, as a negative control, a near absence of mtDNA amplification from whole blood.We screened breast muscle and thigh muscle in 80 birds individually phenotyped for 11 growth and development traits. Substantial individual variation (fivefold) was discovered in both breast and thigh muscle mitochondrial content. Interestingly, across birds we detected a very strong positive relationship between breast and thigh content (correlation coefficient 0.61; P<0.0001), consistent with coordinate regulatory control across the musculature. Further, breast muscle mitochondrial content is negatively correlated with breast muscle yield (-0.27; P=0.037), abdominal fat content (-0.31; P=0.017) and carcass yield (-0.26; P=0.045). Therefore, low breast muscle mitochondrial content is associated with more muscular birds possessing higher abdominal fat, the latter being in line with biomedical models of obesity. Finally, thigh mitochondrial content is negatively correlated with the bow out leg defect (-0.30; P=0.011). Overall, our data point to mitochondrial content as a promising consideration in predictive modelling of production traits.
Keyword Development
Growth
Mitochondria
Muscle
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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