MicroRNA-34 directly targets pair-rule genes and cytoskeleton component in the honey bee

Freitas, Flávia C. P., Pires, Camilla V., Claudianos, Charles, Cristino, Alexandre S. and Simoes, Zilá L. P. (2017) MicroRNA-34 directly targets pair-rule genes and cytoskeleton component in the honey bee. Scientific Reports, 7 . doi:10.1038/srep40884

Author Freitas, Flávia C. P.
Pires, Camilla V.
Claudianos, Charles
Cristino, Alexandre S.
Simoes, Zilá L. P.
Title MicroRNA-34 directly targets pair-rule genes and cytoskeleton component in the honey bee
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2017-01-18
Sub-type Article (original research)
DOI 10.1038/srep40884
Open Access Status DOI
Volume 7
Total pages 10
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Formatted abstract
MicroRNAs (miRNAs) are key regulators of developmental processes, such as cell fate determination and differentiation. Previous studies showed Dicer knockdown in honeybee embryos disrupt the processing of functional mature miRNAs and impairs embryo patterning. Here we investigated the expression profiles of miRNAs in honeybee embryogenesis and the role of the highly conserved miR-34-5p in the regulation of genes involved in insect segmentation. A total of 221 miRNAs were expressed in honey bee embryogenesis among which 97 mature miRNA sequences have not been observed before. Interestingly, we observed a switch in dominance between the 5-prime and 3-prime arm of some miRNAs in different embryonic stages; however, most miRNAs present one dominant arm across all stages of embryogenesis. Our genome-wide analysis of putative miRNA-target networks and functional pathways indicates miR-34-5p is one of the most conserved and connected miRNAs associated with the regulation of genes involved in embryonic patterning and development. In addition, we experimentally validated that miR-34-5p directly interacts to regulatory elements in the 3'-untranslated regions of pair-rule (even-skipped, hairy, fushi-tarazu transcription factor 1) and cytoskeleton (actin5C) genes. Our study suggests that miR-34-5p may regulate the expression of pair-rule and cytoskeleton genes during early development and control insect segmentation.
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Sub-type: Article (original research)
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