Free radical scavenging and angiotensin-I converting enzyme inhibitory peptides from Pacific cod (Gadus macrocephalus) skin gelatine

Ngo, Dai-Hung, Ryu, BoMi, Vo, Thanh-Sang, Himaya, S. W. A. and Kim, Se-Kwon (2011) Free radical scavenging and angiotensin-I converting enzyme inhibitory peptides from Pacific cod (Gadus macrocephalus) skin gelatine. International Journal of Biological Macromolecules, 49 5: 1110-1116. doi:10.1016/j.ijbiomac.2011.09.009


Author Ngo, Dai-Hung
Ryu, BoMi
Vo, Thanh-Sang
Himaya, S. W. A.
Kim, Se-Kwon
Title Free radical scavenging and angiotensin-I converting enzyme inhibitory peptides from Pacific cod (Gadus macrocephalus) skin gelatine
Formatted title
Free radical scavenging and angiotensin-I converting enzyme inhibitory peptides from Pacific cod (Gadus macrocephalus) skin gelatine
Journal name International Journal of Biological Macromolecules   Check publisher's open access policy
ISSN 0141-8130
1879-0003
Publication date 2011-12-01
Sub-type Article (original research)
DOI 10.1016/j.ijbiomac.2011.09.009
Open Access Status Not yet assessed
Volume 49
Issue 5
Start page 1110
End page 1116
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
Potent antioxidative peptides were purified from Pacific cod (Gadus macrocephalus) skin gelatin using alcalase, neutrase, papain, trypsin, pepsin, and α-chymotrypsin. Among them, the papain hydrolysate exhibited the highest antioxidant activity. Therefore, it was further purified and obtained two peptides with amino acid sequences of Thr-Cys-Ser-Pro (388 Da) and Thr-Gly-Gly-Gly-Asn-Val (485.5 Da). The antioxidant activity of the purified peptides was performed by electron spin resonance technique. Moreover, their intracellular free radical scavenging activity using 2′,7′-dichlorofluorescin diacetate and the protective effect against oxidation-induced DNA damage were evaluated in mouse macrophages (RAW 264.7 cells). Furthermore, both peptides have shown potential angiotensin-I converting enzyme inhibitory effect. The present study demonstrated that the peptides derived from Pacific cod (G. macrocephalus) skin gelatin could be used in the food industry as functional ingredients with potent antioxidative and antihypertensive benefits.
Keyword Pacific cod (Gadus macrocephalus) skin gelatin
Bioactive peptides
Antioxidants
RAW 264.7 cells
Angiotensin-I converting enzyme inhibition
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 38 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 44 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 23 Jan 2017, 10:44:00 EST by Himaya Siddhihalu Wickrama Hewage on behalf of Institute for Molecular Bioscience