Peptide isolated from Japanese flounder skin gelatin protects against cellular oxidative damage

Himaya, S. W. A., Ngo, Dai-Hung, Ryu, BoMi and Kim, Se-Kwon (2012) Peptide isolated from Japanese flounder skin gelatin protects against cellular oxidative damage. Journal of Agricultural and Food Chemistry, 60 36: 9112-9119. doi:10.1021/jf302161m


Author Himaya, S. W. A.
Ngo, Dai-Hung
Ryu, BoMi
Kim, Se-Kwon
Title Peptide isolated from Japanese flounder skin gelatin protects against cellular oxidative damage
Journal name Journal of Agricultural and Food Chemistry   Check publisher's open access policy
ISSN 0021-8561
1520-5118
Publication date 2012-08-18
Sub-type Article (original research)
DOI 10.1021/jf302161m
Open Access Status Not yet assessed
Volume 60
Issue 36
Start page 9112
End page 9119
Total pages 8
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Formatted abstract
Gelatin was extracted from the skin of Japanese flounder (Palatichtys olivaceus) and was subjected to enzymatic hydrolysis. The peptic hydrolysate resulted in a potent antioxidative peptide Gly-Gly-Phe-Asp-Met-Gly (582 Da), which bears +12.61 kcal/mol hydrophobicity. The antioxidative potential of the peptide was characterized by analyzing the protective effect of the peptide on reactive oxygen species (ROS)-mediated intracellular macromolecule damage. It was found that the peptide is a potent scavenger of intracellular ROS, thereby protecting the radical-mediated damage of membrane lipids, proteins, and DNA. Moreover, the peptide is capable of upregulating the expression of inherent antioxidative enzymes, superoxide dismutase-1, glutathione, and catalase. Collectively, it can be concluded that Japanese flounder skin, a processing byproduct of filleting, can be effectively used to produce a bioactive peptide with potent antioxidant capacity.
Keyword Antioxidant
Macromolecular oxidation
Palatichtys olivaceus
Peptide
RAW264.7 cells
ROS
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 17 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 23 Jan 2017, 10:21:05 EST by Himaya Siddhihalu Wickrama Hewage on behalf of Institute for Molecular Bioscience