An active peptide purified from gastrointestinal enzyme hydrolysate of Pacific cod skin gelatin attenuates angiotensin-1 converting enzyme (ACE) activity and cellular oxidative stress

Himaya, S. W. A., Ryu, BoMi, Ngo, Dai-Hung and Kim, Se-Kwon (2012) An active peptide purified from gastrointestinal enzyme hydrolysate of Pacific cod skin gelatin attenuates angiotensin-1 converting enzyme (ACE) activity and cellular oxidative stress. Food Chemistry, 132 4: 1872-1882. doi:10.1016/j.foodchem.2011.12.020


Author Himaya, S. W. A.
Ryu, BoMi
Ngo, Dai-Hung
Kim, Se-Kwon
Title An active peptide purified from gastrointestinal enzyme hydrolysate of Pacific cod skin gelatin attenuates angiotensin-1 converting enzyme (ACE) activity and cellular oxidative stress
Journal name Food Chemistry   Check publisher's open access policy
ISSN 0308-8146
1873-7072
Publication date 2012-06-15
Sub-type Article (original research)
DOI 10.1016/j.foodchem.2011.12.020
Open Access Status Not yet assessed
Volume 132
Issue 4
Start page 1872
End page 1882
Total pages 11
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
Seafood processing by-product, Pacific cod (Gadus macrocephalus) skin, was utilised to purify an active peptide with ACE inhibitory and antioxidant activities. Gelatin was extracted from the skin and it was hydrolysed with gastrointestinal endopeptidases (pepsin, trypsin and α-chymotrypsin). Assay-guided purification of the hydrolysate resulted in an active peptide, Leu-Leu-Met-Leu-Asp-Asn-Asp-Leu-Pro-Pro (1301 Da). The peptide showed potent non-competitive ACE inhibition (IC50 = 35.7 μM) and effectively protects cellular macromolecules from reactive oxygen species (ROS) mediated damage. The peptide significantly reduced the oxidation levels of membrane lipids, proteins and DNA in RAW264.7 cells by effectively scavenging the intracellular ROS. Moreover, it was found that the peptide treatment upregulated the m-RNA expression of cellular antioxidative enzymes (superoxide dismutase, glutathione and catalase) and thereby enhanced the intracellular antioxidant mechanisms. These findings suggest that Pacific cod skin could be effectively bioconverted to produce a bioactive peptide, which could be used as a functional food ingredient to control ACE activity and oxidative stress.
Keyword Peptide
ACE inhibitory
Antioxidant
ROS
Antioxidative enzymes
Pacific cod skin
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Mon, 23 Jan 2017, 10:17:05 EST by Himaya Siddhihalu Wickrama Hewage on behalf of Institute for Molecular Bioscience