Clay nanosheets for topical delivery of RNAi for sustained protection against plant viruses

Mitter, Neena, Worrall, Elizabeth A., Robinson, Karl E., Li, Peng, Jain, Ritesh G., Taochy, Christelle, Fletcher, Stephen J., Carroll, Bernard J., Lu, G. Q. (Max) and Xu, Zhi Ping (2017) Clay nanosheets for topical delivery of RNAi for sustained protection against plant viruses. Nature Plants, 3 2: 16207.1-16207.10. doi:10.1038/nplants.2016.207


Author Mitter, Neena
Worrall, Elizabeth A.
Robinson, Karl E.
Li, Peng
Jain, Ritesh G.
Taochy, Christelle
Fletcher, Stephen J.
Carroll, Bernard J.
Lu, G. Q. (Max)
Xu, Zhi Ping
Title Clay nanosheets for topical delivery of RNAi for sustained protection against plant viruses
Journal name Nature Plants   Check publisher's open access policy
ISSN 2055-026X
2055-0278
Publication date 2017-01-09
Sub-type Article (original research)
DOI 10.1038/nplants.2016.207
Open Access Status Not Open Access
Volume 3
Issue 2
Start page 16207.1
End page 16207.10
Total pages 10
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Formatted abstract
Topical application of pathogen-specific double-stranded RNA (dsRNA) for virus resistance in plants represents an attractive alternative to transgenic RNA interference (RNAi). However, the instability of naked dsRNA sprayed on plants has been a major challenge towards its practical application. We demonstrate that dsRNA can be loaded on designer, non-toxic, degradable, layered double hydroxide (LDH) clay nanosheets. Once loaded on LDH, the dsRNA does not wash off, shows sustained release and can be detected on sprayed leaves even 30 days after application. We provide evidence for the degradation of LDH, dsRNA uptake in plant cells and silencing of homologous RNA on topical application. Significantly, a single spray of dsRNA loaded on LDH (BioClay) afforded virus protection for at least 20 days when challenged on sprayed and newly emerged unsprayed leaves. This innovation translates nanotechnology developed for delivery of RNAi for human therapeutics to use in crop protection as an environmentally sustainable and easy to adopt topical spray.
Keyword Virus resistance
RNAi
Naked dsRNA
Spray
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Fri, 13 Jan 2017, 20:34:04 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences