Assessment of the humoral immune response against Plasmodium falciparum rhoptry-associated proteins 1 and 2

Stowers, Anthony, Taylor, Darrin, Prescott, Nicole, Cheng, Qin, Cooper, Juan and Saul, Allan (1997) Assessment of the humoral immune response against Plasmodium falciparum rhoptry-associated proteins 1 and 2. Infection and Immunity, 65 6: 2329-2338.

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Author Stowers, Anthony
Taylor, Darrin
Prescott, Nicole
Cheng, Qin
Cooper, Juan
Saul, Allan
Title Assessment of the humoral immune response against Plasmodium falciparum rhoptry-associated proteins 1 and 2
Formatted title
Assessment of the humoral immune response against Plasmodium falciparum rhoptry-associated proteins 1 and 2
Journal name Infection and Immunity   Check publisher's open access policy
ISSN 0019-9567
1098-5522
Publication date 1997-06-01
Year available 1997
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 65
Issue 6
Start page 2329
End page 2338
Total pages 10
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract Naturally occurring antibody responses to Plasmodium falciparum rhoptry-associated proteins 1 and 2 (RAP-1 and RAP-2) were measured with recombinant and parasite-derived forms of the antigens. For comparative purposes, responses to multiple forms of three other malarial antigens were also examined. The sera of 100 Papua New Guineans were screened for antibodies. Eighty-six and 82% of individuals over 30 years of age had antibodies that recognized parasite-derived RAP-1 and RAP-2, respectively. Importantly, we found that recombinant and native antigens share linear epitopes seen by the human immune system; thus, the recombinant proteins may be adequate human immunogens. However, antibodies affinity purified on recombinant RAP-1 reacted with other antigens in addition to parasite-derived RAP-1. Thus, the antigenicity of RAP-1 may have been overestimated previously. The recognition of RAP-1 and RAP-2 correlated with age and with the recognition of recombinant forms of the ring-infected erythrocyte surface antigen, merozoite surface protein 1, and merozoite surface antigen 2 (MSA2) antigens. Antibodies to these antigens appear to be generated in response to the total exposure to malaria of the host. Antibodies to conserved regions of MSA2 had stronger correlations with both age and the recognition of other antigens than did the full-length recombinant MSA2 molecule. In contrast to results with the other antigens, there was no significant difference in the ages of individuals with a certain antibody titer to the full-length recombinant or parasite-derived MSA2 molecule, but antibodies to these two antigens did correlate with parasitemia. For all antigens tested, antibody levels after two infections can approach the peak levels of antibodies obtained in immune individuals.
Keyword Merozoite surface antigen
Inhibitory monoclonal antibodies
Papua New Guinea
T-Cell
Malaria
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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