Plasmodium falciparum genetic diversity can be characterised using the polymorphic merozoite surface antigen 2 (MSA-2) gene as a single locus marker

Prescott, N, Stowers, AW, Cheng, Q, Bobogare, A, Rzepczyk, CM and Saul, A (1994) Plasmodium falciparum genetic diversity can be characterised using the polymorphic merozoite surface antigen 2 (MSA-2) gene as a single locus marker. Molecular and Biochemical Parasitology, 63 2: 203-212. doi:10.1016/0166-6851(94)90056-6


Author Prescott, N
Stowers, AW
Cheng, Q
Bobogare, A
Rzepczyk, CM
Saul, A
Title Plasmodium falciparum genetic diversity can be characterised using the polymorphic merozoite surface antigen 2 (MSA-2) gene as a single locus marker
Journal name Molecular and Biochemical Parasitology   Check publisher's open access policy
ISSN 0166-6851
Publication date 1994-01-01
Year available 1994
Sub-type Article (original research)
DOI 10.1016/0166-6851(94)90056-6
Open Access Status Not yet assessed
Volume 63
Issue 2
Start page 203
End page 212
Total pages 10
Place of publication AMSTERDAM
Publisher ELSEVIER SCIENCE BV
Language eng
Subject 1312 Molecular Biology
2405 Parasitology
Abstract The genetic diversity of Solomon Island Plasmodium falciparum isolates was examined using MSA-2 as a single locus marker. Amplification of MSA-2 gene fragments showed size polymorphism and the presence of mixed infections. Sequence analysis indicated a global representation of MSA-2 alleles with representatives of 3D7/CAMP allelic subfamilies and the FCQ-27 allelic family being identified. A simplified method of characterisation, utilising PCR-RFLPs of MSA-2 gene fragments, was developed. The RFLPs allowed identification of allelic families and further distinction within the 3D7/CAMP family. The amplification of MSA-2 gene fragments from culture derived lines revealed a loss of diversity for a number of Solomon Island isolates. Genomic diversity was confirmed for Solomon Island lines, along with Papua New Guinean and Thai lines, by the generation of 7H8/6 fingerprints. All lines were distinct and band sharing frequencies and Wagner tree construction failed to identify any geographic clustering.
Keyword DNA fingerprinting
Genetic diversity
Merozoite surface antigen 2
Plasmodium falciparum
Polymerase chain reaction
Restriction fragment length polymorphism
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: ResearcherID Downloads - Archived
Scopus Import - Archived
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 34 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 31 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 12 Jan 2017, 01:01:07 EST by System User