Visualization of fluoride ions in vivo using a gadolinium(III)-coumarin complex-based fluorescence/MRI dual-modal probe

Wang, Yue, Song, Renfeng, Feng, Huan, Guo, Ke, Meng, Qingtao, Chi, Haijun, Zhang, Run and Zhang, Zhiqiang (2016) Visualization of fluoride ions in vivo using a gadolinium(III)-coumarin complex-based fluorescence/MRI dual-modal probe. Sensors, 16 12: 5700-5705. doi:10.3390/s16122165

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Author Wang, Yue
Song, Renfeng
Feng, Huan
Guo, Ke
Meng, Qingtao
Chi, Haijun
Zhang, Run
Zhang, Zhiqiang
Title Visualization of fluoride ions in vivo using a gadolinium(III)-coumarin complex-based fluorescence/MRI dual-modal probe
Journal name Sensors   Check publisher's open access policy
ISSN 1424-8220
1424-8239
Publication date 2016-12-16
Year available 2017
Sub-type Article (original research)
DOI 10.3390/s16122165
Open Access Status DOI
Volume 16
Issue 12
Start page 5700
End page 5705
Total pages 12
Place of publication Basel, Switzerland
Publisher M D P I AG
Language eng
Abstract A new Gadolinium(III)-coumarin complex, DO3A-Gd-CA, was designed and prepared as a dual-modal probe for simultaneous fluorescence and relaxivity responses to fluoride ions (F-) in aqueous media and mice. DO3A-Gd-CA was designed by using Gd(III) center as an MRI signal output unit and fluoride binding site, and the 4-(diethylamino)-coumarin-3-carboxylic acid (CA) as a fluorescence reporter. Upon the addition of fluoride ions to the solution of DO3A-Gd-CA, the liberation of the coordinated CA ligand led to a 5.7-fold fluorescence enhancement and a 75% increase in the longitudinal relaxivity (r₁). The fluorescent detection limit for fluoride ions was determined to be 8 μM based on a 3σ/slope. The desirable features of the proposed DO3A-Gd-CA, such as high sensitivity and specificity, reliability at physiological pH and low cytotoxicity enable its application in visualization of fluoride ion in mice. The successful in vivo imaging indicates that DO3A-Gd-CA could be potentially used in biomedical diagnosis fields.
Formatted abstract
A new Gadolinium(III)–coumarin complex, DO3A-Gd-CA, was designed and prepared as a dual-modal probe for simultaneous fluorescence and relaxivity responses to fluoride ions (F) in aqueous media and mice. DO3A-Gd-CA was designed by using Gd(III) center as an MRI signal output unit and fluoride binding site, and the 4-(diethylamino)-coumarin-3-carboxylic acid (CA) as a fluorescence reporter. Upon the addition of fluoride ions to the solution of DO3A-Gd-CA, the liberation of the coordinated CA ligand led to a 5.7-fold fluorescence enhancement and a 75% increase in the longitudinal relaxivity (r1). The fluorescent detection limit for fluoride ions was determined to be 8 µM based on a 3σ/slope. The desirable features of the proposed DO3A-Gd-CA, such as high sensitivity and specificity, reliability at physiological pH and low cytotoxicity enable its application in visualization of fluoride ion in mice. The successful in vivo imaging indicates that DO3A-Gd-CA could be potentially used in biomedical diagnosis fields.
Keyword Detection
Dual-modal
Fluorescence/MRI
Fluoride ion
Gadolinium(III) complex
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 201602400
122015047
21601076
201610146057
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Australian Institute for Bioengineering and Nanotechnology Publications
 
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