CRIg-expressing peritoneal macrophages are associated with disease severity in patients with cirrhosis and ascites

Irvine, Katharine M., Xuan Banh, Gadd, Victoria L., Wojcik, Kyle K., Ariffin, Juliana K., Jose, Sara, Lukowski, Samuel, Baillie, Gregory J., Sweet, Matthew J. and Powell, Elizabeth E. (2016) CRIg-expressing peritoneal macrophages are associated with disease severity in patients with cirrhosis and ascites. JCI Insight, 1 8: . doi:10.1172/jci.insight.86914

Author Irvine, Katharine M.
Xuan Banh
Gadd, Victoria L.
Wojcik, Kyle K.
Ariffin, Juliana K.
Jose, Sara
Lukowski, Samuel
Baillie, Gregory J.
Sweet, Matthew J.
Powell, Elizabeth E.
Title CRIg-expressing peritoneal macrophages are associated with disease severity in patients with cirrhosis and ascites
Journal name JCI Insight   Check publisher's open access policy
ISSN 2379-3708
Publication date 2016-06-02
Year available 2016
Sub-type Article (original research)
DOI 10.1172/jci.insight.86914
Open Access Status DOI
Volume 1
Issue 8
Total pages 15
Place of publication Ann Arbor, Michigan, United States
Publisher American Society for Clinical Investigation
Language eng
Formatted abstract
Infections are an important cause of morbidity and mortality in patients with decompensated cirrhosis and ascites. Hypothesizing that innate immune dysfunction contributes to susceptibility to infection, we assessed ascitic fluid macrophage phenotype and function. The expression of complement receptor of the immunoglobulin superfamily (CRIg) and CCR2 defined two phenotypically and functionally distinct peritoneal macrophage subpopulations. The proportion of CRIghi macrophages differed between patients and in the same patient over time, and a high proportion of CRIghi macrophages was associated with reduced disease severity (model for end-stage liver disease) score. As compared with CRIglo macrophages, CRIghi macrophages were highly phagocytic and displayed enhanced antimicrobial effector activity. Transcriptional profiling by RNA sequencing and comparison with human macrophage and murine peritoneal macrophage expression signatures highlighted similarities among CRIghi cells, human macrophages, and mouse F4/80hi resident peritoneal macrophages and among CRIglo macrophages, human monocytes, and mouse F4/80lo monocyte-derived peritoneal macrophages. These data suggest that CRIghi and CRIglo macrophages may represent a tissue-resident population and a monocyte-derived population, respectively. In conclusion, ascites fluid macrophage subset distribution and phagocytic capacity is highly variable among patients with chronic liver disease. Regulating the numbers and/or functions of these macrophage populations could provide therapeutic opportunities in cirrhotic patients.
Keyword Spontaneous bacterial peritonitis
Uropathogenic escherichia-coli
Tissue-resident macrophages
Colony-stimulating factor
Complement receptor
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
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