Biotransformation of acyclovir by an enriched nitrifying culture

Xu, Yifeng, Yuan, Zhiguo and Ni, Bing-Jie (2017) Biotransformation of acyclovir by an enriched nitrifying culture. Chemosphere, 170 25-32. doi:10.1016/j.chemosphere.2016.12.014

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ416163_OA.pdf Full text (open access) application/pdf 833.10KB 0

Author Xu, Yifeng
Yuan, Zhiguo
Ni, Bing-Jie
Title Biotransformation of acyclovir by an enriched nitrifying culture
Journal name Chemosphere   Check publisher's open access policy
ISSN 1879-1298
0045-6535
Publication date 2017-03-01
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.chemosphere.2016.12.014
Open Access Status File (Author Post-print)
Volume 170
Start page 25
End page 32
Total pages 8
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Language eng
Subject 1600 Chemistry
2304 Environmental Chemistry
Abstract This work evaluates the biodegradation of the antiviral drug acyclovir by an enriched nitrifying culture during ammonia oxidation and without the addition of ammonium. The study on kinetics was accompanied with the structural elucidation of biotransformation products through batch biodegradation experiments at two different initial levels of acyclovir (15 mg L and 15 μg L). The pseudo first order kinetic studies of acyclovir in the presence of ammonium indicated the higher degradation rates under higher ammonia oxidation rates than those constant degradation rates in the absence of ammonium. The positive correlation was found between acyclovir degradation rate and ammonia oxidation rate, confirming the cometabolism of acyclovir by the enriched nitrifying culture in the presence of ammonium. Formation of the product carboxy-acyclovir (P239) indicated the main biotransformation pathway was aerobic oxidation of the terminal hydroxyl group, which was independent on the metabolic type (i.e. cometabolism or metabolism). This enzyme-linked reaction might be catalyzed by monooxygenase from ammonia oxidizing bacteria or heterotrophs. The formation of carboxy-acyclovir was demonstrated to be irrelevant to the acyclovir concentrations applied, indicating the revealed biotransformation pathway might be the dominant removal pathway of acyclovir in wastewater treatment.
Formatted abstract
This work evaluates the biodegradation of the antiviral drug acyclovir by an enriched nitrifying culture during ammonia oxidation and without the addition of ammonium. The study on kinetics was accompanied with the structural elucidation of biotransformation products through batch biodegradation experiments at two different initial levels of acyclovir (15 mg L−1 and 15 μg L−1). The pseudo first order kinetic studies of acyclovir in the presence of ammonium indicated the higher degradation rates under higher ammonia oxidation rates than those constant degradation rates in the absence of ammonium. The positive correlation was found between acyclovir degradation rate and ammonia oxidation rate, confirming the cometabolism of acyclovir by the enriched nitrifying culture in the presence of ammonium. Formation of the product carboxy-acyclovir (P239) indicated the main biotransformation pathway was aerobic oxidation of the terminal hydroxyl group, which was independent on the metabolic type (i.e. cometabolism or metabolism). This enzyme-linked reaction might be catalyzed by monooxygenase from ammonia oxidizing bacteria or heterotrophs. The formation of carboxy-acyclovir was demonstrated to be irrelevant to the acyclovir concentrations applied, indicating the revealed biotransformation pathway might be the dominant removal pathway of acyclovir in wastewater treatment.
Keyword Acyclovir
Ammonia oxidizing bacteria
Biotransformation
Cometabolism
Nitrification
Wastewater treatment
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID DE130100451
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Advanced Water Management Centre Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 1 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 27 Dec 2016, 10:20:07 EST by System User on behalf of Learning and Research Services (UQ Library)