Assessing the usefulness of clostridia spores for evaluating sewage sludge hygienization

Martín-Díaz, Julia, Ruiz-Hernando, Maria, Astals, Sergi and Lucena, Francisco (2017) Assessing the usefulness of clostridia spores for evaluating sewage sludge hygienization. Bioresource Technology, 225 286-292. doi:10.1016/j.biortech.2016.11.075

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Author Martín-Díaz, Julia
Ruiz-Hernando, Maria
Astals, Sergi
Lucena, Francisco
Title Assessing the usefulness of clostridia spores for evaluating sewage sludge hygienization
Journal name Bioresource Technology   Check publisher's open access policy
ISSN 0960-8524
1873-2976
Publication date 2017-02-01
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.biortech.2016.11.075
Open Access Status File (Author Post-print)
Volume 225
Start page 286
End page 292
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Subject 1502 Bioengineering
2305 Environmental Engineering
2311 Waste Management and Disposal
Abstract The capability of clostridia spores to act as pathogen indicators in sewage sludge treatment was investigated. Sulfite-reducing clostridia and E. coli levels were monitored during waste activated sludge pre-treatments (alkali and ultrasound) and its subsequent mesophilic anaerobic digestion. E. coli was maintained or reduced depending on treatment type and intensity. However, alkali pre-treatment (35.3 gNaOH/kg TS) by itself and alkali (157 gNaOH/kg TS) and ultrasound (27,000 kJ/kg TS) pre-treatments followed by anaerobic digestion provoked reproducible clostridia increases. Specifically, up to 2.7 log after 35.3 gNaOH/kg TS pre-treatment and up to 1.9 and 1.1 log after digesting the 157 gNaOH/kg TS and 27,000 kJ/kg TS pre-treated sludge, respectively. Having rejected the hypotheses of sporulation and floc dissipation, the most plausible explanation for these clostridia increases is re-growth. These results question the suitability of clostridia spores as indicators of sludge treatment and other biological treatments where clostridia may have a role.
Formatted abstract
The capability of clostridia spores to act as pathogen indicators in sewage sludge treatment was investigated. Sulfite-reducing clostridia and E. coli levels were monitored during waste activated sludge pre-treatments (alkali and ultrasound) and its subsequent mesophilic anaerobic digestion. E. coli was maintained or reduced depending on treatment type and intensity. However, alkali pre-treatment (35.3 gNaOH/kg TS) by itself and alkali (157 gNaOH/kg TS) and ultrasound (27,000 kJ/kg TS) pre-treatments followed by anaerobic digestion provoked reproducible clostridia increases. Specifically, up to 2.7 log10 after 35.3 gNaOH/kg TS pre-treatment and up to 1.9 and 1.1 log10 after digesting the 157 gNaOH/kg TS and 27,000 kJ/kg TS pre-treated sludge, respectively. Having rejected the hypotheses of sporulation and floc dissipation, the most plausible explanation for these clostridia increases is re-growth. These results question the suitability of clostridia spores as indicators of sludge treatment and other biological treatments where clostridia may have a role.
Keyword Clostridium spores
Anaerobic digestion
Sewage sludge
Hygienization treatment
Indicator
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID UQFEL1608109
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Advanced Water Management Centre Publications
 
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Created: Thu, 15 Dec 2016, 19:26:08 EST by Sergi Astals-garcia on behalf of Advanced Water Management Centre