Surface rearrangement of adsorbed EGCG–mucin complexes on hydrophilic surfaces

McColl, J., Horvath, R., Yakubov, G. E. and Ramsden, J. J. (2017) Surface rearrangement of adsorbed EGCG–mucin complexes on hydrophilic surfaces. International Journal of Biological Macromolecules, 95 704-712. doi:10.1016/j.ijbiomac.2016.11.108

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Author McColl, J.
Horvath, R.
Yakubov, G. E.
Ramsden, J. J.
Title Surface rearrangement of adsorbed EGCG–mucin complexes on hydrophilic surfaces
Journal name International Journal of Biological Macromolecules   Check publisher's open access policy
ISSN 0141-8130
1879-0003
Publication date 2017-02-01
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.ijbiomac.2016.11.108
Open Access Status File (Author Post-print)
Volume 95
Start page 704
End page 712
Total pages 9
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2017
Language eng
Abstract The kinetic adsorption–desorption behaviour of porcine gastric mucin in the presence of physiologically relevant concentrations of the polyphenol epigallocatechin gallate (EGCG) was investigated using high-resolution kinetic optical waveguide lightmode spectroscopy (OWLS) and atomic force microscopy (AFM). Comparison with dynamic light scattering results from EGCG–mucin mixtures indicates that discrete particles are formed whose size increases with increasing EGCG:mucin ratio. These particles are deduced to be the adsorbing entities, which fuse on the surface to form complex surface layers. At low molar EGCG:mucin ratios (<∼1000), aggregates fuse on the surface to form a monolayer similar to one of pure mucin. With increasing EGCG concentration, the surface assembly of aggregates becomes consistent with their rearrangement and spreading in the shape of a spherical segment. At the highest molar ratios investigated (>12,000) the particles begin to destabilize. The presence of EGCG leads to birefringence hysteresis during adsorption–desorption, indicating structural rearrangement, even at molar ratios ∼1000. The intensification of the phenomenon with increasing EGCG:mucin ratio mimics what was previously observed with the increase of mucin concentration in an EGCG-free system.
Keyword Glycoprotein
Polyphenol
Clustering
OWLS
Kinetics
Hysteresis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Chemical Engineering Publications
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Created: Mon, 12 Dec 2016, 22:31:18 EST by Gleb Yakubov on behalf of School of Chemical Engineering