Evidence for possible interactions between PLP and DM20 within the myelin sheath

Mclaughlin, M, Hunter, DJB, Thomson, CE, Yool, D, Kirkham, D, Freer, AA and Griffiths, IR (2002) Evidence for possible interactions between PLP and DM20 within the myelin sheath. GLIA, 39 1: 31-36. doi:10.1002/glia.10091

Author Mclaughlin, M
Hunter, DJB
Thomson, CE
Yool, D
Kirkham, D
Freer, AA
Griffiths, IR
Title Evidence for possible interactions between PLP and DM20 within the myelin sheath
Journal name GLIA   Check publisher's open access policy
ISSN 0894-1491
Publication date 2002-01-01
Year available 2002
Sub-type Article (original research)
DOI 10.1002/glia.10091
Open Access Status
Volume 39
Issue 1
Start page 31
End page 36
Total pages 6
Place of publication NEW YORK
Publisher WILEY-LISS
Language eng
Subject 2808 Neurology
2804 Cellular and Molecular Neuroscience
Abstract PLP and its smaller DM20 isoform constitute the major proteins of CNS myelin. Previous studies indicated a role for the proteins in maintaining the intraperiod line of the myelin sheath and the integrity of axons and suggested that both isoforms were necessary to provide these functions. The present study shows that each isoform is capable individually of inserting into compact myelin. Employing chromatographic extraction procedures designed to maintain the natural conformation of the proteins we found that most PLP and DM20 remained associated. Using an antibody specific to the PLP isoform, we were able to co-immunoprecipitate DM20 from the major fraction of the extracted equine myelin and from mouse native whole myelin. We suggest that PLP and DM20 may form a hetero-oligomeric complex within the myelin sheath, probably in association with specific lipids and that this arrangement is essential for the normal structure of myelin and axons.
Keyword Co-immunoprecipitation
Intraperiod line
Transgenic complementation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 15 times in Scopus Article | Citations
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