High-rate, high temperature acetotrophic methanogenesis governed by a three population consortium in anaerobic bioreactors

Ho, Dang, Jensen, Paul, Gutierrez-Zamora, Maria-Luisa, Beckmann, Sabrina, Manefield, Mike and Batstone, Damien (2016) High-rate, high temperature acetotrophic methanogenesis governed by a three population consortium in anaerobic bioreactors. PLoS ONE, 11 8: . doi:10.1371/journal.pone.0159760


Author Ho, Dang
Jensen, Paul
Gutierrez-Zamora, Maria-Luisa
Beckmann, Sabrina
Manefield, Mike
Batstone, Damien
Title High-rate, high temperature acetotrophic methanogenesis governed by a three population consortium in anaerobic bioreactors
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2016-08-04
Sub-type Article (original research)
DOI 10.1371/journal.pone.0159760
Open Access Status DOI
Volume 11
Issue 8
Total pages 13
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2017
Language eng
Formatted abstract
A combination of acetate oxidation and acetoclastic methanogenesis has been previously identified to enable high-rate methanogenesis at high temperatures (55 to 65° C), but this capability had not been linked to any key organisms. This study combined RNA-stable isotope probing on 13C-labelled acetate and 16S amplicon sequencing to identify the active micro-organisms involved in high-rate methanogenesis. Active biomass was harvested from three bench-scale thermophilic bioreactors treating waste activated sludge at 55, 60 and 65° C, and fed with 13-C labelled and 12C-unlabelled acetate. Acetate uptake and cumulative methane production were determined and kinetic parameters were estimated using model-based analysis. Pyrosequencing performed on 13C- enriched samples indicated that organisms accumulating labelled carbon were Coprothermobacter (all temperatures between 55 and 65° C), acetoclastic Methanosarcina (55 to 60° C) and hydrogenotrophic Methanothermobacter (60 to 65° C). The increased relative abundance of Coprothermobacter with increased temperature corresponding with a shift to syntrophic acetate oxidation identified this as a potentially key oxidiser. Methanosarcina likely acts as both a hydrogen utilising and acetoclastic methanogen at 55° C, and is replaced by Methanothermobacter as a hydrogen utiliser at higher temperatures.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Advanced Water Management Centre Publications
 
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