An ancient role for nitric oxide in regulating the animal pelagobenthic life cycle: evidence from a marine sponge

Ueda, Nobuo, Richards, Gemma S., Degnan, Bernard M., Kranz, Alexandrea, Adamska, Maja, Croll, Roger P. and Degnan, Sandie M. (2016) An ancient role for nitric oxide in regulating the animal pelagobenthic life cycle: evidence from a marine sponge. Scientific Reports, 6 37546: . doi:10.1038/srep37546


Author Ueda, Nobuo
Richards, Gemma S.
Degnan, Bernard M.
Kranz, Alexandrea
Adamska, Maja
Croll, Roger P.
Degnan, Sandie M.
Title An ancient role for nitric oxide in regulating the animal pelagobenthic life cycle: evidence from a marine sponge
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2016-11-22
Year available 2016
Sub-type Article (original research)
DOI 10.1038/srep37546
Open Access Status DOI
Volume 6
Issue 37546
Total pages 14
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Formatted abstract
In many marine invertebrates, larval metamorphosis is induced by environmental cues that activate sensory receptors and signalling pathways. Nitric oxide (NO) is a gaseous signalling molecule that regulates metamorphosis in diverse bilaterians. In most cases NO inhibits or represses this process, although it functions as an activator in some species. Here we demonstrate that NO positively regulates metamorphosis in the poriferan Amphimedon queenslandica. High rates of A. queenslandica metamorphosis normally induced by a coralline alga are inhibited by an inhibitor of nitric oxide synthase (NOS) and by a NO scavenger. Consistent with this, an artificial donor of NO induces metamorphosis even in the absence of the alga. Inhibition of the ERK signalling pathway prevents metamorphosis in concert with, or downstream of, NO signalling; a NO donor cannot override the ERK inhibitor. NOS gene expression is activated late in embryogenesis and in larvae, and is enriched in specific epithelial and subepithelial cell types, including a putative sensory cell, the globular cell; DAF-FM staining supports these cells being primary sources of NO. Together, these results are consistent with NO playing an activating role in induction of A. queenslandica metamorphosis, evidence of its highly conserved regulatory role in metamorphosis throughout the Metazoa.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
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