Cell-type-specific expression of NFIX in the developing and adult cerebellum

Fraser, James, Essebier, Alexandra, Gronostajski, Richard M., Boden, Mikael, Wainwright, Brandon J., Harvey, Tracey J. and Piper, Michael (2016) Cell-type-specific expression of NFIX in the developing and adult cerebellum. Brain Structure and Function, 222 5: 1-20. doi:10.1007/s00429-016-1340-8


Author Fraser, James
Essebier, Alexandra
Gronostajski, Richard M.
Boden, Mikael
Wainwright, Brandon J.
Harvey, Tracey J.
Piper, Michael
Title Cell-type-specific expression of NFIX in the developing and adult cerebellum
Journal name Brain Structure and Function   Check publisher's open access policy
ISSN 1863-2661
1863-2653
Publication date 2016-01-01
Year available 2016
Sub-type Article (original research)
DOI 10.1007/s00429-016-1340-8
Open Access Status Not yet assessed
Volume 222
Issue 5
Start page 1
End page 20
Total pages 20
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract Transcription factors from the nuclear factor one (NFI) family have been shown to play a central role in regulating neural progenitor cell differentiation within the embryonic and post-natal brain. NFIA and NFIB, for instance, promote the differentiation and functional maturation of granule neurons within the cerebellum. Mice lacking Nfix exhibit delays in the development of neuronal and glial lineages within the cerebellum, but the cell-type-specific expression of this transcription factor remains undefined. Here, we examined the expression of NFIX, together with various cell-type-specific markers, within the developing and adult cerebellum using both chromogenic immunohistochemistry and co-immunofluorescence labelling and confocal microscopy. In embryos, NFIX was expressed by progenitor cells within the rhombic lip and ventricular zone. After birth, progenitor cells within the external granule layer, as well as migrating and mature granule neurons, expressed NFIX. Within the adult cerebellum, NFIX displayed a broad expression profile, and was evident within granule cells, Bergmann glia, and interneurons, but not within Purkinje neurons. Furthermore, transcriptomic profiling of cerebellar granule neuron progenitor cells showed that multiple splice variants of Nfix are expressed within this germinal zone of the post-natal brain. Collectively, these data suggest that NFIX plays a role in regulating progenitor cell biology within the embryonic and post-natal cerebellum, as well as an ongoing role within multiple neuronal and glial populations within the adult cerebellum.
Keyword NFIX
Cerebellum
External granular layer
Granule neuron
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 1057751
DP160100368
C026714
FT120100170
Institutional Status UQ

 
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