MALDI-TOF MS meets WGS in a VRE outbreak investigation

Schlebusch, S., Price, G. R., Gallagher, R. L., Horton-Szar, V., Elbourne, L. D. H., Griffin, P., Venter, D. J., Jensen, S. O. and van Hal, S. J. (2016) MALDI-TOF MS meets WGS in a VRE outbreak investigation. European Journal of Clinical Microbiology and Infectious Diseases, 36 3: 1-5. doi:10.1007/s10096-016-2824-4


Author Schlebusch, S.
Price, G. R.
Gallagher, R. L.
Horton-Szar, V.
Elbourne, L. D. H.
Griffin, P.
Venter, D. J.
Jensen, S. O.
van Hal, S. J.
Title MALDI-TOF MS meets WGS in a VRE outbreak investigation
Journal name European Journal of Clinical Microbiology and Infectious Diseases   Check publisher's open access policy
ISSN 1435-4373
0934-9723
Publication date 2016-11-26
Sub-type Article (original research)
DOI 10.1007/s10096-016-2824-4
Open Access Status Not yet assessed
Volume 36
Issue 3
Start page 1
End page 5
Total pages 5
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract The use of MALDI-TOF MS (matrix-assisted laser desorption/ ionization-time of flight mass spectrometry) and WGS (whole genome sequencing) has been described for identification and strain relatedness determination. We describe the complementary use of MALDI-TOF MS and WGS in a VRE (vancomycin-resistant enterococci) outbreak investigation, and discuss some of the challenges with defining strain similarity across these two platforms. Although both assays indicated multiple clusters involved in the outbreak of vancomycin resistant Enterococcus faecium isolates from positive blood cultures of four haematology-oncology patients, the small cohort and discrepancies between findings indicate the limitations of MALDI-TOF MS and the cautious interpretation of MALDI-TOF MS dendrograms during outbreaks. For definitive determination of the evolutionary distance between isolates, WGS can be used.
Formatted abstract
The use of MALDI-TOF MS (matrix-assisted laser desorption/ ionization–time of flight mass spectrometry) and WGS (whole genome sequencing) has been described for identification and strain relatedness determination. We describe the complementary use of MALDI-TOF MS and WGS in a VRE (vancomycin-resistant enterococci) outbreak investigation, and discuss some of the challenges with defining strain similarity across these two platforms. Although both assays indicated multiple clusters involved in the outbreak of vancomycin resistant Enterococcus faecium isolates from positive blood cultures of four haematology–oncology patients, the small cohort and discrepancies between findings indicate the limitations of MALDI-TOF MS and the cautious interpretation of MALDI-TOF MS dendrograms during outbreaks. For definitive determination of the evolutionary distance between isolates, WGS can be used.
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