High-speed biosensing strategy for non-invasive profiling of multiple cancer fusion genes in urine

Koo, Kevin M., Wee, Eugene J. H. and Trau, Matt (2017) High-speed biosensing strategy for non-invasive profiling of multiple cancer fusion genes in urine. Biosensors and Bioelectronics, 89 2: 715-720. doi:10.1016/j.bios.2016.11.024


Author Koo, Kevin M.
Wee, Eugene J. H.
Trau, Matt
Title High-speed biosensing strategy for non-invasive profiling of multiple cancer fusion genes in urine
Journal name Biosensors and Bioelectronics   Check publisher's open access policy
ISSN 1873-4235
0956-5663
Publication date 2017-03-15
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.bios.2016.11.024
Open Access Status Not yet assessed
Volume 89
Issue 2
Start page 715
End page 720
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Subject 1305 Biotechnology
1304 Biophysics
2204 Biomedical Engineering
1603 Electrochemistry
Abstract Aberrant chromosal rearrangements, such as the multiple variants of TMPRSS2:ERG fusion gene mutations in prostate cancer (PCa), are promising diagnostic and prognostic biomarkers due to their specific expression in cancerous tissue only. Additionally, TMPRSS2:ERG variants are detectable in urine to provide non-invasive PCa diagnostic sampling as an attractive surrogate for needle biopsies. Therefore, rapid and simplistic assays for identifying multiple urinary TMPRSS2:ERG variants are potentially useful to aid in early cancer detection, immediate patient risk stratification, and prompt personalized treatment. However, current strategies for simultaneous detection of multiple gene fusions are limited by tedious and prolonged experimental protocols, thus limiting their use as rapid clinical screening tools. Herein, we report a simple and rapid gene fusion strategy which expliots the specificity of DNA ligase and the speed of isothermal amplification to simultaneously detect multiple fusion gene RNAs within a short sample-to-answer timeframe of 60 min. The method has a low detection limit of 2 amol (1000 copies), and was successfully applied for non-invasive fusion gene profiling in patient urine samples with subsequent validation by a PCR-based gold standard approach.
Keyword Gene fusion biomarkers
Non-invasive detection
Prostate cancer cell lines
Recombinase polymerase amplification
Urine samples
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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