Characterization of a bioactive acyclotide from Palicourea rigida

Pinto, Michelle F. S., Silva, Osmar N., Viana, Juilan C., Porto, William F., Migliolo, Ludovico, da Cunha, Nicolau B., Gomes Jr., Nelson, Fensterseifer, Isabel C. M., Colgrave, Michelle L., Craik, David J., Dias, Simoni C. and Franco, Octavio L. (2016) Characterization of a bioactive acyclotide from Palicourea rigida. Journal of Natural Products, 79 11: 2767-2773. doi:10.1021/acs.jnatprod.6b00270


Author Pinto, Michelle F. S.
Silva, Osmar N.
Viana, Juilan C.
Porto, William F.
Migliolo, Ludovico
da Cunha, Nicolau B.
Gomes Jr., Nelson
Fensterseifer, Isabel C. M.
Colgrave, Michelle L.
Craik, David J.
Dias, Simoni C.
Franco, Octavio L.
Title Characterization of a bioactive acyclotide from Palicourea rigida
Formatted title
Characterization of a bioactive acyclotide from Palicourea rigida
Journal name Journal of Natural Products   Check publisher's open access policy
ISSN 0163-3864
1520-6025
Publication date 2016-11-23
Sub-type Article (original research)
DOI 10.1021/acs.jnatprod.6b00270
Open Access Status Not yet assessed
Volume 79
Issue 11
Start page 2767
End page 2773
Total pages 7
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Formatted abstract
The extraction and purification of parigidin-br3, a cyclotide analogue belonging to the “bracelet” subfamily, from Palicourea rigida leaves is discussed. Unlike conventional cyclotides, parigidin-br3 has free N- and C-termini, as identified by MALDI-TOF/TOF analysis and confirmed by gene structure elucidation, and is one of a small number of acyclotides discovered during recent years. Parigidin-br3 showed cytotoxic activity against MCF-7 (breast cancer) and CACO2 (colorectal adenocarcinoma) cells, with IC50 values of ∼2.5 μM and less than 10% hemolytic activity. Overall, parigidin-br3 is a promising new molecule with cytotoxic properties against tumor cell lines and, unlike many synthetic acyclic analogues, demonstrates that cytotoxic activity is not limited to conventional (i.e., cyclic) cyclotides.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
 
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Created: Thu, 01 Dec 2016, 00:36:18 EST by Susan Allen on behalf of Institute for Molecular Bioscience