Prepartum body condition score and plane of nutrition affect the hepatic transcriptome during the transition period in grazing dairy cows

Vailati-Riboni, M., Meier, S., Burke, C. R., Kay, J. K., Mitchell, M. D., Walker, C. G., Crookenden, M. A., Heiser, A., Rodriguez-Zas, S. L., Roche, J. R. and Loor, J. J. (2016) Prepartum body condition score and plane of nutrition affect the hepatic transcriptome during the transition period in grazing dairy cows. Bmc Genomics, 17 1: . doi:10.1186/s12864-016-3191-3


Author Vailati-Riboni, M.
Meier, S.
Burke, C. R.
Kay, J. K.
Mitchell, M. D.
Walker, C. G.
Crookenden, M. A.
Heiser, A.
Rodriguez-Zas, S. L.
Roche, J. R.
Loor, J. J.
Title Prepartum body condition score and plane of nutrition affect the hepatic transcriptome during the transition period in grazing dairy cows
Journal name Bmc Genomics   Check publisher's open access policy
ISSN 1471-2164
Publication date 2016-11-02
Sub-type Article (original research)
DOI 10.1186/s12864-016-3191-3
Open Access Status DOI
Volume 17
Issue 1
Total pages 15
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Background: A transcriptomic approach was used to evaluate potential interactions between prepartum body condition score (BCS) and feeding management in the weeks before calving on hepatic metabolism during the periparturient period.

Methods: Thirty-two mid-lactation grazing dairy cows of mixed age and breed were randomly allocated to one of four treatment groups in a 2×2 factorial arrangement: two prepartum BCS categories [4.0 (thin, BCS4) and 5.0 (optimal, BCS5); based on a 10-point scale], and two levels of energy intake during the 3weeks preceding calving (75 and 125% of estimated requirements). Liver samples were obtained at 7, 7, and 28 d relative to parturition and subsequent RNA was hybridized to the Agilent 44K Bovine (V2) Microarray chip. The Dynamic Impact Approach was used for pathway analysis, and Ingenuity Pathway Analysis was used for gene network analysis.

Results: The greater number of differentially expressed genes in BCS4 cows in response to prepartum feed allowance (1071 vs 310, over the entire transition period) indicates that these animals were more responsive to prepartum nutrition management than optimally-conditioned cows. However, independent of prepartum BCS, pathway analysis revealed that prepartal feeding level had a marked effect on carbohydrate, amino acid, lipid, and glycan metabolism. Altered carbohydrate and amino acid metabolism suggest a greater and more prolonged negative energy balance postpartum in BCS5 cows overfed prepartum. This is supported by opposite effects of prepartum feeding in BCS4 compared with BCS5 cows in pathways encompassing amino acid, vitamin, and co-factor metabolism. The prepartum feed restriction ameliorates the metabolic adaptation to the onset of lactation in BCS5 cows, while detrimentally affecting BCS4 cows, which seem to better adapt when overfed. Alterations in the glycosaminoglycans synthesis pathway support this idea, indicating better hepatic health status in feed-restricted BCS5 and overfed BCS4 cows. Furthermore, IPA network analysis suggests liver damage in feed-restricted thin cows, likely due to metabolic overload.

Conclusion: Overall, the data support the hypothesis that overfeeding in late-pregnancy should be limited to underconditioned cows, while cows with optimal degree of body condition should be maintained on an energy-restricted diet.
Keyword Peripartum
BCS
Prepartum nutrition
Liver transcriptome
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
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