Purification of the keratan sulfate proteoglycan expressed in prostatic secretory cells and its identification as lumican

Holland, John W., Meehan, Katie L., Redmond, Sharon L. and Dawkins, Hugh J. S. (2004) Purification of the keratan sulfate proteoglycan expressed in prostatic secretory cells and its identification as lumican. Prostate, 59 3: 252-259. doi:10.1002/pros.20002


Author Holland, John W.
Meehan, Katie L.
Redmond, Sharon L.
Dawkins, Hugh J. S.
Title Purification of the keratan sulfate proteoglycan expressed in prostatic secretory cells and its identification as lumican
Journal name Prostate   Check publisher's open access policy
ISSN 0270-4137
1097-0045
Publication date 2004-05-15
Sub-type Article (original research)
DOI 10.1002/pros.20002
Open Access Status DOI
Volume 59
Issue 3
Start page 252
End page 259
Total pages 8
Place of publication New York
Publisher Wiley-Liss, Inc.
Language eng
Abstract BACKGROUND. Secretory epithelial cells of human prostate contain a keratan sulfate proteoglycan (KSPG) associated with the prostatic secretory granules (PSGs). The proteoglycan has not been identified, but like the PSGs, it is lost in the early stages of malignant transformation. METHODS. Anion exchange and affinity chromatography were used to purify KSPG from human prostate tissue. Enzymatic deglycosylation was used to remove keratan sulfate (KS). The core protein was isolated using 2D gel electrophoresis, digested in-gel with trypsin, and identified by peptide mass fingerprinting (PMF). RESULTS. The purified proteoglycan was detected as a broad smear on Western blots with an apparent molecular weight of 65-95 kDa. The KS moiety was susceptible to digestion with keratanase 11 and peptide N-glycosidase F defining it as highly sulfated and N-linked to the core protein. The core protein was identified, following deglycosylation and PMF, as lumican and subsequently confirmed by Western blotting using an anti-lumican antibody. CONCLUSIONS. The KSPG associated with PSGs in normal prostate epithelium is lumican. While the role of lumican in extracellular matrix is well established, its function in the prostate secretory process is not known. It's potential to facilitate packaging of polyamines in PSGs, to act as a tumor suppressor and to mark the early stages of malignant transformation warrant further investigation. (C) 2003 Wiley-Liss, Inc.
Keyword Endocrinology & Metabolism
Urology & Nephrology
2d Electrophoresis
Peptide Mass Fingerprinting
Tumor Marker
Human Breast-carcinoma
Cancer Cells
Gene
Adenocarcinoma
Benign
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 14 Aug 2007, 00:15:34 EST