Axon guidance studies using a microfluidics-based chemotropic gradient generator

Pujic, Zac, Nguyen, Huyen, Glass, Nick, Cooper-White, Justin and Goodhill, Geoffrey J. (2016). Axon guidance studies using a microfluidics-based chemotropic gradient generator. In 2nd (Ed.), Chemotaxis: methods and protocols (pp. 273-285) New York, NY United States: Springer New York. doi:10.1007/978-1-4939-3480-5_20


Author Pujic, Zac
Nguyen, Huyen
Glass, Nick
Cooper-White, Justin
Goodhill, Geoffrey J.
Title of chapter Axon guidance studies using a microfluidics-based chemotropic gradient generator
Title of book Chemotaxis: methods and protocols
Place of Publication New York, NY United States
Publisher Springer New York
Publication Year 2016
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-4939-3480-5_20
Open Access Status Not yet assessed
Year available 2016
Series Methods in Molecular Biology
ISBN 9781493934782
9781493934805
ISSN 1064-3745
Editor 2nd
Volume number 1407
Chapter number 20
Start page 273
End page 285
Total pages 13
Total chapters 27
Language eng
Abstract/Summary Microfluidics can be used to generate flow-driven gradients of chemotropic guidance cues with precisely controlled steepnesses for indefinite lengths of time. Neuronal cells grown in the presence of these gradients can be studied for their response to the effects exerted by the cues. Here we describe a polydimethylsiloxane (PDMS) microfluidics chamber capable of producing linear gradients of soluble factors, stable for at least 18 h, suitable for axon guidance studies. Using this device we demonstrate turning of superior cervical ganglion axons by gradients of nerve growth factor (NGF). The chamber produces robust gradients, is inexpensive to mass produce, can be mounted on a tissue culture dish or glass coverslip for long term time-lapse microscopy imaging, and is suitable for immunostaining.
Keyword Gradient
Chemotropic cue
Microfluidic
Axon guidance
Chemotaxis
Nerve growth factor
Superior cervical ganglion
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Thu, 24 Nov 2016, 02:10:49 EST by Kirstie Asmussen on behalf of Queensland Brain Institute