Isolation of two insecticidal toxins from venom of the Australian theraphosid spider Coremiocnemis tropix

Ikonomopoulou, Maria P., Smith, Jennifer J., Herzig, Volker, Pineda, Sandy S., Dziemborowicz, Sławomir, Er, Sing-Yan, Durek, Thomas, Gilchrist, John, Alewood, Paul F., Nicholson, Graham M., Bosmans, Frank and King, Glenn F. (2016) Isolation of two insecticidal toxins from venom of the Australian theraphosid spider Coremiocnemis tropix. Toxicon, 123 62-70. doi:10.1016/j.toxicon.2016.10.013

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Author Ikonomopoulou, Maria P.
Smith, Jennifer J.
Herzig, Volker
Pineda, Sandy S.
Dziemborowicz, Sławomir
Er, Sing-Yan
Durek, Thomas
Gilchrist, John
Alewood, Paul F.
Nicholson, Graham M.
Bosmans, Frank
King, Glenn F.
Title Isolation of two insecticidal toxins from venom of the Australian theraphosid spider Coremiocnemis tropix
Formatted title
Isolation of two insecticidal toxins from venom of the Australian theraphosid spider Coremiocnemis tropix
Journal name Toxicon   Check publisher's open access policy
ISSN 1879-3150
0041-0101
Publication date 2016-12-01
Sub-type Article (original research)
DOI 10.1016/j.toxicon.2016.10.013
Open Access Status File (Author Post-print)
Volume 123
Start page 62
End page 70
Total pages 9
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Collection year 2017
Language eng
Formatted abstract
Sheep flystrike is caused by parasitic flies laying eggs on soiled wool or open wounds, after which the hatched maggots feed on the sheep flesh and often cause large lesions. It is a significant economic problem for the livestock industry as infestations are difficult to control due to ongoing cycles of larval development into flies followed by further egg laying. We therefore screened venom fractions from the Australian theraphosid spider Coremiocnemis tropix to identify toxins active against the sheep blowfly Lucilia cuprina, which is the primary cause of flystrike in Australia. This screen led to isolation of two insecticidal peptides, Ct1a and Ct1b, that are lethal to blowflies within 24 h of injection. The primary structure of these peptides was determined using a combination of Edman degradation and sequencing of a C. tropix venom-gland transcriptome. Ct1a and Ct1b contain 39 and 38 amino acid residues, respectively, including six cysteine residues that form three disulfide bonds. Recombinant production in bacteria (Escherichia coli) resulted in low yields of Ct1a whereas solid-phase peptide synthesis using native chemical ligation produced sufficient quantities of Ct1a for functional analyses. Synthetic Ct1a had no effect on voltage-gated sodium channels from the American cockroach Periplanata americana or the German cockroach Blattella germanica, but it was lethal to sheep blowflies with an LD50 of 1687 pmol/g.
Keyword Bioinsecticide
Coremiocnemis tropix
Flystrike
Insecticidal peptide
Lucilia cuprina
Spider venom
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
 
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