Double adjuvanting strategy for peptide-based vaccines: trimethyl chitosan nanoparticles for lipopeptide delivery

Marasini, Nirmal, Giddam, Ashwini K., Khalil, Zeinab G., Hussein, Waleed M., Capon, Robert J., Batzloff, Michael R., Good, Michael F., Toth, Istvan and Skwarczynski, Mariusz (2016) Double adjuvanting strategy for peptide-based vaccines: trimethyl chitosan nanoparticles for lipopeptide delivery. Nanomedicine, 11 24: 3223-3235. doi:10.2217/nnm-2016-0291


Author Marasini, Nirmal
Giddam, Ashwini K.
Khalil, Zeinab G.
Hussein, Waleed M.
Capon, Robert J.
Batzloff, Michael R.
Good, Michael F.
Toth, Istvan
Skwarczynski, Mariusz
Title Double adjuvanting strategy for peptide-based vaccines: trimethyl chitosan nanoparticles for lipopeptide delivery
Journal name Nanomedicine   Check publisher's open access policy
ISSN 1743-5889
1748-6963
Publication date 2016-12-01
Year available 2016
Sub-type Article (original research)
DOI 10.2217/nnm-2016-0291
Open Access Status Not Open Access
Volume 11
Issue 24
Start page 3223
End page 3235
Total pages 13
Place of publication London, United Kingdom
Publisher Future Medicine
Language eng
Abstract Aim: To develop novel polymer-based nanoscale delivery system for lipopeptide-based vaccine against group A Streptococcus (GAS). Materials & methods: Four types of lipopeptide antigen-loaded polymeric nanoparticles (NP) were prepared. NP were accessed for their capacity to be taken up by dendritic cells; effect on dendritic cell maturation; ability to induce mucosal and systemic immunity; and capability to induce antibody responses that opsonize GAS bacteria. Results & discussion: The combination of adjuvanting properties of lipopeptides and dextran/trimethyl chitosan-based NP had a synergistic effect on humoral immunity, and the produced antibodies showed high opsonic activity against clinical GAS isolates. Conclusion: Biocompatible NP-bearing trimethyl chitosan and dextran are efficient as mucosal adjuvants for the intranasal delivery of lipopeptide-based vaccines.
Formatted abstract
Aim: To develop novel polymer-based nanoscale delivery system for lipopeptide-based vaccine against group A Streptococcus (GAS).

Materials & methods: Four types of lipopeptide antigen-loaded polymeric nanoparticles (NP) were prepared. NP were accessed for their capacity to be taken up by dendritic cells; effect on dendritic cell maturation; ability to induce mucosal and systemic immunity; and capability to induce antibody responses that opsonize GAS bacteria.

Results & discussion: The combination of adjuvanting properties of lipopeptides and dextran/trimethyl chitosan-based NP had a synergistic effect on humoral immunity, and the produced antibodies showed high opsonic activity against clinical GAS isolates.

Conclusion: Biocompatible NP-bearing trimethyl chitosan and dextran are efficient as mucosal adjuvants for the intranasal delivery of lipopeptide-based vaccines.
Keyword Biotechnology & Applied Microbiology
Nanoscience & Nanotechnology
Biotechnology & Applied Microbiology
Science & Technology - Other Topics
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 496600
Institutional Status UQ

 
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Created: Sat, 19 Nov 2016, 01:32:57 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences