Abnormal anterior corneal morphology in diabetes observed using in vivo laser-scanning confocal microscopy

Dehghani, Cirous, Pritchard, Nicola, Edwards, Katie, Russell, Anthony W., Malik, Rayaz A. and Efron, Nathan (2016) Abnormal anterior corneal morphology in diabetes observed using in vivo laser-scanning confocal microscopy. Ocular Surface, 14 4: 507-514. doi:10.1016/j.jtos.2016.07.004


Author Dehghani, Cirous
Pritchard, Nicola
Edwards, Katie
Russell, Anthony W.
Malik, Rayaz A.
Efron, Nathan
Title Abnormal anterior corneal morphology in diabetes observed using in vivo laser-scanning confocal microscopy
Journal name Ocular Surface   Check publisher's open access policy
ISSN 1937-5913
1542-0124
Publication date 2016-10-01
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.jtos.2016.07.004
Open Access Status Not yet assessed
Volume 14
Issue 4
Start page 507
End page 514
Total pages 8
Place of publication Philadelphia, PA United States
Publisher Elsevier
Language eng
Abstract To assess if diabetes alters corneal epithelial, anterior stromal and subbasal nerve plexus morphology and to determine the associations between these and other clinical variables.
Formatted abstract
Purpose

To assess if diabetes alters corneal epithelial, anterior stromal and subbasal nerve plexus morphology and to determine the associations between these and other clinical variables.

Methods

A cohort of 78 participants with diabetes (39 with Type 1 and 39 with Type 2 diabetes) and 29 age-matched healthy controls underwent laser-scanning confocal microscopy of the central cornea. Intermediate cell density (ICD), basal cell density (BCD), anterior stromal cell density (ASCD), corneal nerve fiber density (CNFD) and nerve fiber length (CNFL) were quantified.

Results

Compared with controls, participants with diabetes showed reduced ICD (6097 ± 669 vs. 5548 ± 669 no/mm2, P<.01), BCD (8925 ± 1196 vs. 7842 ± 1040 cell/mm2, P<.01), CNFD (23.4 ± 9.1 vs. 17.5 ± 9.7 no/mm2, P<.01) and CNFL (21.0 ± 4.0 vs. 17.4 ± 4.9 mm/mm2, P<.01), with no difference in ASCD (785 ± 262 vs. 733 ± 278 cell/mm2, respectively, P=.40). None of these structural parameters were associated with type of diabetes (P>.06). Multiple regression analysis showed that ICD and BCD were inversely associated with the diabetes duration and diastolic blood pressure (P<.05) and positively associated with CNFD (P<.01). CNFD and CNFL were inversely associated with HbA1c (P<.01), while ASCD was inversely associated with age (P<.01).

Conclusions

Corneal epithelial cells and subbasal nerve fibers are reduced in patients with diabetes; however, anterior stromal cells show no difference. Furthermore, abnormalities in corneal epithelial cells and nerves are interrelated and correlated with modifiable risk factors.
Keyword Corneal epithelium
Corneal nerves
Corneal stroma
Diabetes
Laser-scanning confocal microscopy
Risk factors
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Admin Only - School of Medicine
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 1 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 08 Nov 2016, 11:04:41 EST by System User on behalf of School of Medicine