Transcriptome analysis of grey mullet (Mugil cephalus) after challenge with Lactococcus garvieae

Byadgi, Omkar, Chen, Yao-Chung, Barnes, Andrew C., Tsai, Ming-An, Wang, Pei-Chyi and Chen, Shih-Chu (2016) Transcriptome analysis of grey mullet (Mugil cephalus) after challenge with Lactococcus garvieae. Fish and Shellfish Immunology, 58 593-603. doi:10.1016/j.fsi.2016.10.006

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Author Byadgi, Omkar
Chen, Yao-Chung
Barnes, Andrew C.
Tsai, Ming-An
Wang, Pei-Chyi
Chen, Shih-Chu
Title Transcriptome analysis of grey mullet (Mugil cephalus) after challenge with Lactococcus garvieae
Formatted title
Transcriptome analysis of grey mullet (Mugil cephalus) after challenge with Lactococcus garvieae
Journal name Fish and Shellfish Immunology   Check publisher's open access policy
ISSN 1095-9947
1050-4648
Publication date 2016-11-01
Sub-type Article (original research)
DOI 10.1016/j.fsi.2016.10.006
Open Access Status File (Author Post-print)
Volume 58
Start page 593
End page 603
Total pages 11
Place of publication London, United Kingdom
Publisher Academic Press
Collection year 2017
Language eng
Formatted abstract
Grey mullet (Mugil cephalus) is an economically important fish species in Taiwan mariculture industry. Moreover, grey mullet are common hosts of a bacterial infection by Lactococcus garvieae. However, until now the information related to the immune system of grey mullet is unclear. Therefore, to understand the molecular basis underlying the host immune response to L. garvieae infection, Illumina HiSeq™ 2000 was used to analyse the head kidney and spleen transcriptome of infected grey mullet. De novo assembly of paired-end reads yielded 55,203 unigenes. Comparative analysis of the expression profiles between bacterial challenge fish and control fish identified a total of 7192 from head kidney and 7280 in spleen differentially expressed genes (P < 0.05), including 4211 upregulated genes and 2981 downregulated genes in head kidney, while in spleen 3598 genes were upregulated and 3682 downregulated. A significant enrichment analysis of these differentially expressed genes (DEG) in spleen and head kidney revealed major immune-related pathways, including complement and coagulation cascades, Toll-like receptor signalling, and antigen processing and presentation. Moreover, selected DEGs were validated using qPCR. Altogether, the results obtained on immune-related genes may allow for a better understanding of immunity in grey mullet to Lactococcus garvieae, carrying out detailed functional analysis of these genes and developing strategies for efficient immune protection against infections in grey mullet.
Keyword Grey mullet (Mugil cephalus)
Illumina paired-end sequencing
Immune response
Lactococcus garvieae
Transcriptome
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biological Sciences Publications
Centre for Marine Studies Publications
 
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Created: Tue, 08 Nov 2016, 10:36:16 EST by System User on behalf of Learning and Research Services (UQ Library)