Intensity of mutualism breakdown is determined by temperature not amplification of Wolbachia genes

Rohrscheib, Chelsie E., Frentiu, Francesca D., Horn, Emilie, Ritchie, Fiona K., van Swinderen, Bruno, Weible, Michael W., O'Neill, Scott L. and Brownlie, Jeremy C. (2016) Intensity of mutualism breakdown is determined by temperature not amplification of Wolbachia genes. PLoS Pathogens, 12 9: . doi:10.1371/journal.ppat.1005888


Author Rohrscheib, Chelsie E.
Frentiu, Francesca D.
Horn, Emilie
Ritchie, Fiona K.
van Swinderen, Bruno
Weible, Michael W.
O'Neill, Scott L.
Brownlie, Jeremy C.
Title Intensity of mutualism breakdown is determined by temperature not amplification of Wolbachia genes
Formatted title
Intensity of mutualism breakdown is determined by temperature not amplification of Wolbachia genes
Journal name PLoS Pathogens   Check publisher's open access policy
ISSN 1553-7374
1553-7366
Publication date 2016-09-23
Sub-type Article (original research)
DOI 10.1371/journal.ppat.1005888
Open Access Status DOI
Volume 12
Issue 9
Total pages 15
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Formatted abstract
Wolbachia are maternally transmitted intracellular bacterial symbionts that infect approximately 40% of all insect species. Though several strains of Wolbachia naturally infect Drosophila melanogaster and provide resistance against viral pathogens, or provision metabolites during periods of nutritional stress, one virulent strain, wMelPop, reduces fly lifespan by half, possibly as a consequence of over-replication. While the mechanisms that allow wMelPop to over-replicate are still of debate, a unique tandem repeat locus in the wMelPop genome that contains eight genes, referred to as the “Octomom” locus has been identified and is thought to play an important regulatory role. Estimates of Octomom locus copy number correlated increasing copy number to both Wolbachia bacterial density and increased pathology. Here we demonstrate that infected fly pathology is not dependent on an increased Octomom copy number, but does strongly correlate with increasing temperature. When measured across developmental time, we also show Octomom copy number to be highly variable across developmental time within a single generation. Using a second pathogenic strain of Wolbachia, we further demonstrate reduced insect lifespan can occur independently of a high Octomom locus copy number. Taken together, this data demonstrates that the mechanism/s of wMelPop virulence is more complex than has been previously described.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Brain Institute Publications
 
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