Mouse fetal germ cell isolation and culture techniques

Spiller, Cassy M., Burnet, Guillaume and Bowles, Josephine (2017). Mouse fetal germ cell isolation and culture techniques. In Michael Buszczak (Ed.), Germline stem cells Second edition ed. (pp. 173-183) New York, NY, United States: Humana Press. doi:10.1007/978-1-4939-4017-2_13


Author Spiller, Cassy M.
Burnet, Guillaume
Bowles, Josephine
Title of chapter Mouse fetal germ cell isolation and culture techniques
Title of book Germline stem cells
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2017
Sub-type Chapter in reference work, encyclopaedia, manual or handbook
DOI 10.1007/978-1-4939-4017-2_13
Series Methods in molecular biology
Edition Second edition
ISBN 9781493940172
9781493940158
ISSN 1064-3745
1940-6029
Editor Michael Buszczak
Volume number 1463
Chapter number 13
Start page 173
End page 183
Total pages 11
Total chapters 16
Language eng
Formatted Abstract/Summary
The fetal gonad contains a great variety of differentiating cell populations, of which germ cells make up a small percentage. In order to study germ cell-specific gene and protein expression, as well as determine direct effects of signaling molecules, it is necessary to prepare enriched populations of germ cells and maintain them in culture for several hours to multiple days. The protocols in this chapter are designed to provide a guide for the isolation or enrichment of mouse primordial germ cells (from 9.5 days postcoitum (dpc) to 18.5 dpc) by flow cytometry (Subheading 3.1) or magnetic sorting (Subheading 3.2), followed by primary germ cell culture (Subheading 3.3).
Keyword FACS
Germ cells
MACS
Magnetic sorting
Serum-free
Q-Index Code BX
Q-Index Status Provisional Code
Institutional Status UQ

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Created: Mon, 24 Oct 2016, 21:26:39 EST by Cassy Spiller on behalf of School of Biomedical Sciences