Comparative visual ecology of cephalopods from different habitats

Chung, Wen-Sung and Marshall, N. Justin (2016) Comparative visual ecology of cephalopods from different habitats. Proceedings of the Royal Society B: Biological Sciences, 283 1838: . doi:10.1098/rspb.2016.1346


Author Chung, Wen-Sung
Marshall, N. Justin
Title Comparative visual ecology of cephalopods from different habitats
Journal name Proceedings of the Royal Society B: Biological Sciences   Check publisher's open access policy
ISSN 1471-2954
0962-8452
Publication date 2016-09-14
Sub-type Article (original research)
DOI 10.1098/rspb.2016.1346
Open Access Status Not yet assessed
Volume 283
Issue 1838
Total pages 10
Place of publication London, United Kingdom
Publisher Royal Society of London
Language eng
Subject 2700 Medicine
2400 Immunology and Microbiology
1300 Biochemistry, Genetics and Molecular Biology
2300 Environmental Science
1100 Agricultural and Biological Sciences
Abstract Previous investigations of vision and visual pigment evolution in aquatic predators have focused on fish and crustaceans, generally ignoring the cephalopods. Since the first cephalopod opsin was sequenced in late 1980s, we now have data on over 50 cephalopod opsins, prompting this functional and phylogenetic examination. Much of this data does not specifically examine the visual pigment spectral absorbance position (λ) relative to environment or lifestyle, and cephalopod opsin functional adaptation and visual ecology remain largely unknown. Here we introduce a new protocol for photoreceptor microspectrophotometry (MSP) that overcomes the difficulty of bleaching the bistable visual pigment and that reveals eight coastal coleoid cephalopods to be monochromatic with λ varying from 484 to 505 nm. A combination of current MSP results, the λ values previously characterized using cephalopod retinal extracts (467-500 nm) and the corresponding opsin phylogenetic tree were used for systematic comparisons with an end goal of examining the adaptations of coleoid visual pigments to different light environments. Spectral tuning shifts are described in response to different modes of life and light conditions. A new spectral tuning model suggests that nine amino acid substitution sites may determine the direction and the magnitude of spectral shifts.
Formatted abstract
Previous investigations of vision and visual pigment evolution in aquatic predators have focused on fish and crustaceans, generally ignoring the cephalopods. Since the first cephalopod opsin was sequenced in late 1980s, we now have data on over 50 cephalopod opsins, prompting this functional and phylogenetic examination. Much of this data does not specifically examine the visual pigment spectral absorbance position (λmax) relative to environment or lifestyle, and cephalopod opsin functional adaptation and visual ecology remain largely unknown. Here we introduce a new protocol for photoreceptor microspectrophotometry (MSP) that overcomes the difficulty of bleaching the bistable visual pigment and that reveals eight coastal coleoid cephalopods to be monochromatic with λmax varying from 484 to 505 nm. A combination of current MSP results, the λmax values previously characterized using cephalopod retinal extracts (467-500 nm) and the corresponding opsin phylogenetic tree were used for systematic comparisons with an end goal of examining the adaptations of coleoid visual pigments to different light environments. Spectral tuning shifts are described in response to different modes of life and light conditions. A new spectral tuning model suggests that nine amino acid substitution sites may determine the direction and the magnitude of spectral shifts.
Keyword Cephalopod
Microspectrophotometry
Opsin
Spectral tuning
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Brain Institute Publications
 
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