Effect of carbon sources on the aggregation of photo fermentative bacteria induced by L-cysteine for enhancing hydrogen production

Xie, Guo-Jun, Liu, Bing-Feng, Ding, Jie, Wang, Qilin, Ma, Chao, Zhou, Xu and Ren, Nan-Qi (2016) Effect of carbon sources on the aggregation of photo fermentative bacteria induced by L-cysteine for enhancing hydrogen production. Environmental Science and Pollution Research, 23 24: 1-11. doi:10.1007/s11356-016-7756-0


Author Xie, Guo-Jun
Liu, Bing-Feng
Ding, Jie
Wang, Qilin
Ma, Chao
Zhou, Xu
Ren, Nan-Qi
Title Effect of carbon sources on the aggregation of photo fermentative bacteria induced by L-cysteine for enhancing hydrogen production
Journal name Environmental Science and Pollution Research   Check publisher's open access policy
ISSN 1614-7499
0944-1344
Publication date 2016-09-30
Sub-type Article (original research)
DOI 10.1007/s11356-016-7756-0
Open Access Status Not yet assessed
Volume 23
Issue 24
Start page 1
End page 11
Total pages 11
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Subject 2304 Environmental Chemistry
2310 Pollution
2307 Health, Toxicology and Mutagenesis
Abstract Poor flocculation of photo fermentative bacteria resulting in continuous biomass washout from photobioreactor is a critical challenge to achieve rapid and stable hydrogen production. In this work, the aggregation of Rhodopseudomonas faecalis RLD-53 was successfully developed in a photobioreactor and the effects of different carbon sources on hydrogen production and aggregation ability were investigated. Extracellular polymeric substances (EPS) production by R. faecalis RLD-53 cultivated using different carbon sources were stimulated by addition of L-cysteine. The absolute ζ potentials of R. faecalis RLD-53 were considerably decreased with addition of L-cysteine, and aggregation barriers based on DLVO dropped to 15–43 % of that in control groups. Thus, R. faecalis RLD-53 flocculated effectively, and aggregation abilities of strain RLD-53 cultivated with acetate, propionate, lactate and malate reached 29.35, 32.34, 26.07 and 24.86 %, respectively. In the continuous test, hydrogen-producing activity was also promoted and reached 2.45 mol H/mol lactate, 3.87 mol H/mol propionate and 5.10 mol H/mol malate, respectively. Therefore, the aggregation of R. faecalis RLD-53 induced by L-cysteine is independent on the substrate types, which ensures the wide application of this technology to enhance hydrogen recovery from wastewater dominated by different organic substrates.
Formatted abstract
Poor flocculation of photo fermentative bacteria resulting in continuous biomass washout from photobioreactor is a critical challenge to achieve rapid and stable hydrogen production. In this work, the aggregation of Rhodopseudomonas faecalis RLD-53 was successfully developed in a photobioreactor and the effects of different carbon sources on hydrogen production and aggregation ability were investigated. Extracellular polymeric substances (EPS) production by R. faecalis RLD-53 cultivated using different carbon sources were stimulated by addition of L-cysteine. The absolute ζ potentials of R. faecalis RLD-53 were considerably decreased with addition of L-cysteine, and aggregation barriers based on DLVO dropped to 15–43 % of that in control groups. Thus, R. faecalis RLD-53 flocculated effectively, and aggregation abilities of strain RLD-53 cultivated with acetate, propionate, lactate and malate reached 29.35, 32.34, 26.07 and 24.86 %, respectively. In the continuous test, hydrogen-producing activity was also promoted and reached 2.45 mol H2/mol lactate, 3.87 mol H2/mol propionate and 5.10 mol H2/mol malate, respectively. Therefore, the aggregation of R. faecalis RLD-53 induced by L-cysteine is independent on the substrate types, which ensures the wide application of this technology to enhance hydrogen recovery from wastewater dominated by different organic substrates.
Keyword Bioaggregate
Carbon source
Hydrogen production
L-cysteine
Rhodopseudomonas faecalis RLD-53
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 51478139
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Advanced Water Management Centre Publications
 
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