Development of marker genes for jasmonic acid signaling in shoots and roots of wheat

Liu, Hongwei, Carvalhais, Lilia Costa, Kazan, Kemal and Schenk, Peer M. (2016) Development of marker genes for jasmonic acid signaling in shoots and roots of wheat. Plant Signaling and Behavior, 11 5: . doi:10.1080/15592324.2016.1176654


Author Liu, Hongwei
Carvalhais, Lilia Costa
Kazan, Kemal
Schenk, Peer M.
Title Development of marker genes for jasmonic acid signaling in shoots and roots of wheat
Journal name Plant Signaling and Behavior   Check publisher's open access policy
ISSN 1559-2324
1559-2316
Publication date 2016-05-03
Year available 2016
Sub-type Article (original research)
DOI 10.1080/15592324.2016.1176654
Open Access Status Not yet assessed
Volume 11
Issue 5
Total pages 8
Place of publication Philadelphia, PA United States
Publisher Taylor & Francis
Language eng
Formatted abstract
The jasmonic acid (JA) signaling pathway plays key roles in a diverse array of plant development, reproduction, and responses to biotic and abiotic stresses. Most of our understanding of the JA signaling pathway derives from the dicot model plant Arabidopsis thaliana, while corresponding knowledge in wheat is somewhat limited. In this study, the expression of 41 genes implicated in the JA signaling pathway has been assessed on 10 day-old bread wheat seedlings, 24 h, 48 h, and 72 h after methyl-jasmonate (MeJA) treatment using quantitative real-time PCR. The examined genes have been previously reported to be involved in JA biosynthesis and catabolism, JA perception and signaling, and pathogen defense in wheat shoots and roots. This study provides evidence to suggest that the effect of MeJA treatment is more prominent in shoots than roots of wheat seedlings, and substantial regulation of the JA pathway-dependent defense genes occurs at 72 h after MeJA treatment. Results show that the expression of 22 genes was significantly affected by MeJA treatment in wheat shoots. However, only PR1.1 and PR3 were significantly differentially expressed in wheat roots, both at 24 h post-MeJA treatment, with other genes showing large variation in their gene expression in roots. While providing marker genes on JA signaling in wheat, future work may focus on elucidating the regulatory function of JA-modulated transcription factors, some of which have well-studied potential orthologs in Arabidopsis.
Keyword Jasmonate
Marker genes
PR genes
Transcription factor
Wheat
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 2 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 11 Oct 2016, 13:16:33 EST by System User on behalf of School of Agriculture and Food Sciences