Analyzing cell death by nuclear staining with Hoechst 33342

Crowley, Lisa C., Marfell, Brooke J. and Waterhouse, Nigel J. (2016) Analyzing cell death by nuclear staining with Hoechst 33342. Cold Spring Harbor Protocols, 2016 9: 778-781. doi:10.1101/pdb.prot087205

Author Crowley, Lisa C.
Marfell, Brooke J.
Waterhouse, Nigel J.
Title Analyzing cell death by nuclear staining with Hoechst 33342
Journal name Cold Spring Harbor Protocols   Check publisher's open access policy
ISSN 1559-6095
Publication date 2016-09-01
Year available 2016
Sub-type Article (original research)
DOI 10.1101/pdb.prot087205
Open Access Status Not yet assessed
Volume 2016
Issue 9
Start page 778
End page 781
Total pages 4
Place of publication Cold Spring Harbor, NY, United States
Publisher Cold Spring Harbor Laboratory Press
Language eng
Abstract The nuclei of healthy cells are generally spherical, and the DNA is evenly distributed. During apoptosis the DNA becomes condensed, but this process does not occur during necrosis. Nuclear condensation can therefore be used to distinguish apoptotic cells from healthy cells or necrotic cells. Dyes that bind to DNA, such as Hoechst 33342 or 4′,6-diamidino-2-phenylindole (DAPI), can be used to observe nuclear condensation. These dyes fluoresce at 461 nm when excited by ultraviolet light and can therefore be visualized using conventional fluorescent microscopes equipped with light sources that emit light at ∼350 nm and filter sets that permit the transmission of light at ∼460 nm. This protocol describes staining and visualization of cells stained with Hoechst 33342, but it can be adapted for staining with DAPI or other dyes.
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ

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Sub-type: Article (original research)
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