Morphological analysis of cell death by cytospinning followed by rapid staining

Crowley, Lisa C., Marfell, Brooke J. and Waterhouse, Nigel J. (2016) Morphological analysis of cell death by cytospinning followed by rapid staining. Cold Spring Harbor Protocols, 2016 9: 773-777. doi:10.1101/pdb.prot087197


Author Crowley, Lisa C.
Marfell, Brooke J.
Waterhouse, Nigel J.
Title Morphological analysis of cell death by cytospinning followed by rapid staining
Journal name Cold Spring Harbor Protocols   Check publisher's open access policy
ISSN 1559-6095
1940-3402
Publication date 2016-09-01
Sub-type Article (original research)
DOI 10.1101/pdb.prot087197
Open Access Status Not yet assessed
Volume 2016
Issue 9
Start page 773
End page 777
Total pages 5
Place of publication Cold Spring Harbor, NY, United States
Publisher Cold Spring Harbor Laboratory Press
Language eng
Abstract Identifying and characterizing different forms of cell death can be facilitated by staining internal cellular structures with dyes such as hematoxylin and eosin (H&E). These dyes stain the nucleus and cytoplasm, respectively, and optimized reagents (e.g., Rapi-Diff, Rapid Stain, or Quick Dip) are commonly used in pathology laboratories. Fixing and staining adherent cells with these optimized reagents is a straightforward procedure, but apoptotic cells may detach from the culture plate and be washed away during the fixing and staining procedure. To prevent the loss of apoptotic cells, cells can be gently centrifuged onto glass slides by cytospinning before fixing and staining. In addition to apoptotic cells, this procedure can be used on cells in suspension, or adherent cells that have been trypsinized and removed from the culture dish. This protocol describes cytospinning followed by Rapi- Diff staining for morphological analysis of cell death.
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 2 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 11 Oct 2016, 11:23:17 EST by System User on behalf of Learning and Research Services (UQ Library)